Fig. 5
Verification of rapid-cycling Brassica rapa transformation. a Schematic of T-DNA and flanking region of the circular binary vector p201G, with corresponding aph, GFP and backbone PCR products depicted at bottom. b representative results of PCR assays; +, GFP-positive; −, GFP-negative; Agro, Agrobacterium strain GV3101(pMP90)(p201G); WT, wild-type RCBr plant; VI, T2 from vacuum infiltration; FD, T2 from floral dip; M, marker. C58 glyA is an Agrobacterium chromosomal gene. c Southern blot analysis. Left, electrophoresis gel of BamHI-digested plant genomic DNA; right, Southern blot probed with GFP probe indicated in (a)