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Fig. 10 | BMC Plant Biology

Fig. 10

From: Genome-wide identification of cyclophilin genes in Gossypium hirsutum and functional characterization of a CYP with antifungal activity against Verticillium dahliae

Fig. 10

Inhibition of mycelial growth of V. dahliae by GhCYP-3. a, Inhibition of mycelial growth of V. dahliae by GhCYP-3 recombinant protein. The inhibition results were observed at 5 days after incubation. Clearing inhibition zones were formed around the disc containing recombinant GhCYP-3 (1 & 2). The disc containing water was as control (3 & 4). b and c, Extracts from transgenic plants significantly reducing the number of V. dahliae colonies. V. dahliae spores were inoculated with plant extracts and then be spread on PDA plates. After 48 h, the number of fungal colonies on plates contained transgenes extracts was drastically less than the H2O and WT. Data represent mean ± SE of three biological replicates. Asterisks indicate statistically significant differences as determined by Dunnett's multiple comparisons test (*P < 0.05, **P < 0.01, ***P < 0.001)

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