Skip to main content
Fig. 8 | BMC Plant Biology

Fig. 8

From: Genome-wide identification of cyclophilin genes in Gossypium hirsutum and functional characterization of a CYP with antifungal activity against Verticillium dahliae

Fig. 8

Genetic transformations of Arabidopsis with GhCYP-3 and identification of transgenic lines. a, Schematic representation of pBI121-GhCYP-3 vector. b, Kanamycin resistance screening of positive seedlings; c, PCR analysis of genomic DNA from Kanamycin resistant lines. d, Semi-qPCR confirmed expression of GhCYP-3 in transgenic lines. AtACT2 (AT3G18780) was used as the internal standard. e, PPIase activity in wild-type (WT) and two transgenic lines. Data represent mean ± SE (n> 20) from three independent experiments. Asterisks indicate significant differences between WT and transgenic lines (Sidak's multiple comparisons test; ns= no significant, *P<0.05, **P<0.01). M, marker; L1 and L2, transgenic Arabidopsis lines; P, plasmid of pBI121-GhCYP-3 as positive control

Back to article page

BMC Plant Biology

ISSN: 1471-2229

Contact us