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Table 2 Monosaccharide composition of the different sequential extracts of M. sativa stems

From: Does long-term cadmium exposure influence the composition of pectic polysaccharides in the cell wall of Medicago sativa stems?

Average mol percentage (mol %)

Fraction

Fuc

Ara

Rha

Gal

Glc

Xyl

Man

GalA

GlcA

H2O

Ctr

0.45 ± 2.74−2

36.63 ± 1.46

1.96 ± 1.16−1*

22.49 ± 4.22− 1

12.30 ± 7.37− 1

4.07 ± 3.01− 1*

7.75 ± 7.06− 1

13.70 ± 1.2

0.67 ± 9.04− 2

Cd

0.50 ± 1.87− 2

34.56 ± 8.14− 1

2.53 ± 1.69− 1*

23.55 ± 4.86− 1

10.54 ± 3.06− 1

5.21 ± 3.21− 1*

7.99 ± 3.95− 1

14.61 ± 7.07− 1

0.51 ± 6.16− 2

EDTA

Ctr

0.81 ± 2.13− 2

52.81 ± 1.48*

3.65 ± 1.34− 1*

15.94 ± 2.66− 1

1.96 ± 7.97− 2*

4.77 ± 2.91− 1*

4.16 ± 1.38− 1

15.37 ± 1.18*

0.52 ± 6.4− 2

Cd

0.77 ± 2.47− 2

44.67 ± 6.82− 1*

4.26 ± 2.2− 1*

15.77 ± 3.23− 1

1.68 ± 4.82− 2*

6.61 ± 1.02–1*

4.27 ± 1.69− 1

21.30 ± 6.99− 1*

0.68 ± 1.71− 1

1 M KOH

Ctr

0.23 ± 2.29− 2

4.21 ± 4.25− 1

1.18 ± 1.18− 1

2.32 ± 2.64− 1

2.37 ± 2.95− 1

73.11 ± 9.95− 1

1.10 ± 8.91− 2

15.29 ± 2.5− 1

0.20 ± 9.69− 3

Cd

0.25 ± 3.2−2

4.28 ± 5.36− 1

1.49 ± 2.05− 1

2.63 ± 3.54− 1

2.05 ± 2.61− 1

71.36 ± 1.67

1.69 ± 4.05− 1

16.04 ± 8.22− 1

0.21 ± 3.74− 2

4 M KOH

Ctr

1.76 ± 7.38− 1

6.23 ± 2.63

0.44 ± 1.53–1*

5.60 ± 5.12− 1*

10.74 ± 6.21

53.50 ± 2.56

11.67 ± 3.92

9.41 ± 1.93

0.65 ± 5.6− 1

Cd

1.65 ± 3.41−2

6.21 ± 1.25− 1

0.70 ± 7.72− 2*

6.30 ± 5.68− 2*

15.01 ± 7.45− 1

49.81 ± 2.11

11.78 ± 2.09

7.72 ± 5.24−1

0.82 ± 2.15− 1

CWP

Ctr

0.54 ± 1.58−2

11.93 ± 3.18−1*

1.65 ± 5.74− 2*

13.63 ± 2.97− 1

20.50 ± 3.78− 1

36.52 ± 7.1− 1

3.62 ± 1.06− 1*

11.17 ± 2.17− 1

0.43 ± 2.8− 2

Cd

0.60 ± 4.26− 2

17.00 ± 1.76*

2.14 ± 1.18− 1*

14.54 ± 1.44

20.72 ± 2.08

30.58 ± 4.8

2.84 ± 1.86− 1*

11.22 ± 5.11− 1

0.37 ± 5.98− 2

  1. SEM standard error of the mean, Ctr control, Fuc fucose, Ara arabinose, Rha rhamnose, Gal galactose, Glc glucose, Xyl xylose, Man mannose, GalA galacturonic acid, GlcA glucuronic acid; CWP cell wall pellet
  2. Values are given as an average from five biological replicates ± SEM. Significant changes were determined with a t-test (p-value ≤0.05) and are indicated with an asterisk