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Fig. 9 | BMC Plant Biology

Fig. 9

From: Genome-wide characterization of aspartic protease (AP) gene family in Populus trichocarpa and identification of the potential PtAPs involved in wood formation

Fig. 9

N-glycosylation analysis of PtAP proteins. a N-glycosylation site analysis of PtAP proteins from the categories A, B and C. The asterisk denotes the predicted N-glycosylation sites. Identical and conserved residues are shaded in black and gray, respectively. b Semi-qRT-PCR analysis of PtAP17 and PtAP66 expression in wild type (WT) and transgenic Populus. PtActin2 was used as a loading control. c Protein extracts were isolated from the leaves of PtAP17-OE4 and PtAP66-OE1 transgenic Populus, respectively. After PNGase F treatments (with or without), protein extracts were separated using 10% SDS-PAGE and followed by immunoblotting with anti-FLAG antibody

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