Fig. 3
Detection of CsCRUC alleles with CRISPR-mediated mutations in T2 generation plant lines using a ddPCR drop-off assay. a Fluorescence intensity plot from wild type camelina displaying double-positive droplets for both the drop-off probe (FAM) and reference probe (HEX). Plant_gRNA512–13-10 and plant_gRNA512–1-69 are representative lines containing CRISPR-mediated mutations and generate droplets that are double-positive (FAM and HEX) and droplets that are single-positive (HEX). b Quantification of drop-off probe events and reference probe events in wild type and nine T2 plants containing mutations. Probe targets are normalized to 100 reference probe events. Values are an average of three replicates ± S.D. Differences between expected reference probe events and observed reference probe events were assessed using a chi-square test. c Fractional abundance (%) of mutated alleles from b