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Fig. 1 | BMC Plant Biology

Fig. 1

From: Interactional similarities and differences in the protein complex of PCNA and DNA replication factor C between rice and Arabidopsis

Fig. 1

Yeast-two-hybrid assay to assess physical interactions between PCNA and RFC subunits of Arabidopsis and rice. The co-transformed strains are spotted on SD-Leu-Trp (a) and SD-Leu-Trp-His-Ade (b) plates to test the physical interactions between the candidate proteins. Yeast strains co-transformed with the ‘empty’ AD or BD plasmids are used as negative controls. AD, pGADT7 vector; BK, pGBKT7 vector; SD, synthetic dextrose

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