Fig. 5
From: Deposition of a cutin apoplastic barrier separating seed maternal and zygotic tissues
Expression of cutin-related genes in tt16 and tt1 seeds. a and b RT-qPCR analyses of a set of genes involved in cutin deposition in tt16 ovules (0 DAF) and seeds (2 and 4 DAF) (a) and tt1seeds (4 DAF) (b). Values are relative to wild type. Error bars represent standard deviations. Asterisks indicate statistical difference between mutant and wild type at the same time point (Student’s t test, *: P < 0.05; **: P < 0.01; ***: P < 0.001). Student’s t test for ATT1 in tt16 background at 4DAF: P = 0.0652. c and d GUS activity in cleared whole mounts of ProMYB30:gMYB30-uidA (c) and ProMYB30:gMYB30-uidA;tt16 (d) seeds at two-cell embryo stage. Ecotype Col. Scale bars: 30 μm