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Fig. 6 | BMC Plant Biology

Fig. 6

From: Deposition of a cutin apoplastic barrier separating seed maternal and zygotic tissues

Fig. 6

TT1 expression in the integuments. a to f Fluorescence images of ProTT1:NTF ovules (a to c) and seeds (d to f). Green, NTF fluorescence; purple, a to e propidium iodide, f auto-fluorescence. Ecotype Col. g to n RNA in situ hybridization analyses of wild type and tt1–4 mutant ovules and seeds with TT1 and HIS4 antisense probes. (g) and (h) Wild type ovules at 0 DAF. (i) and (j) tt1–4 ovules at 0 DAF. (k) to (m) Wild type seeds at 1 DAF. (n) tt1–4 seed at 1 DAF. Ecotype Ws. Scale bars: (a) and (b) 20 μm, (c) and (d) 30 μm, (e) to (n) 50 μm

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