Fig. 1

Cloning and characterization of GhWIN2. (a) Phylogenetic analysis of WIN proteins from cotton (Gossypium hirsutum, Gh), rice (Oryza sativa, Os), barley (Hordeum vulgare, Hv), tomato (Solanum lycopersicum, Sl), and Arabidopsis (At). Accession numbers: OsWR1(Os02g0202000); OsWR2(AK061163); HvHUD (KP245804.1); AtWIN1/SHN1(AT1g15360); AtWIN2/SHN2(At5g11190); AtWIN3/SHN3(At5g25390); SlSHN1(XP_004235965.1); SlSHN2(XP_004251719.1); SlSHN3(NM_001319202). The GhWIN2 is indicated by a red box. The rooted neighbor-joining tree was based on multiple sequence alignment using the MEGA5.1 software. (b) Subcellular localization of GhWIN2. GFP and DAPI fluorescence in the cotyledons of five-day-old plants of line 4 (scale bars: 10 μm). (C, D) The expression of GhWIN2 in two-week-old cotton seedlings after treatment with 100 μM ABA (c) and 100 mM NaCl (d). The mock samples were treated with double distilled water. Data were from three independent replicates and the results are means ± SD. The relative transcription levels were normalized using GhUBQ7. Asterisks indicate significant differences between treated and mock plants according to Student’s t-test (* P < 0.05; ** P < 0.01)