Fig. 3

GhWIN2 activation of the promoter of the GhCYP86A4 gene. (a) Luminescence signal of transient co-expression of GhWIN2/GhCYP86A4_pro:LUC or GhWIN2^V-A/GhCYP86A4_pro:LUC on Nicotiana benthamiana leaves. The photos were taken 2 days after infiltration with the same amount of Agrobacterium tumefaciens cultures containing the specified constructs. Two sites were injected for every treatment. (b) Sequence alignments of AP2/EREBP proteins. The conserved valine is marked in red. GenBank accession numbers: Oryza sativa OsDREB1A (AAN02486), OsDREB2A (AAN02487), AtDREB1A (Q9M0L0), Limonium bicolor LbDREB (ACP18975), Populus euphratica PeDREB2 (ABL86587), Hordeum vulgare HvNUD (AKF40403). (c, d) Expression of LUC transcripts 2 days after co-infiltration of Agrobacterium cells harboring GhWIN2/GhCYP86A4_pro:LUC (c) and GhWIN2^V-A/GhCYP86A4_pro:LUC (d). Data represent means ± SD from three independent biological replicates; Student’s t-test; * P < 0.05, ** P < 0.01