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Fig. 4 | BMC Plant Biology

Fig. 4

From: Outlook for coeliac disease patients: towards bread wheat with hypoimmunogenic gluten by gene editing of α- and γ-gliadin gene families

Fig. 4

Acid-PAGE of selected Paragon γ-irradiated mutant lines that showed changes in gliadin protein profiles. Gliadin extracts from grains of the M4 generation of Paragon γ-irradiated mutant population were run on non-denaturing Acid-PAGE alongside a gliadin extract from Paragon (Paragon WT). Each panel represent a different gel. The lanes displayed next to each other have been run alongside each other. The black arrows point at the changes observed in the irradiated lines. a and b α-gliadin bands missing, probably correlated to changes in Chr6-AS, c and d α-gliadin bands changes that are different from any change observed in deletion lines and nullisomic/tetrasomic lines, e γ- and ω-gliadin bands missing, probably due to changes in 1BS, f γ-gliadin bands missing and ω-gliadin bands shifts and intensity changes, probably due to changes in Chr1-AS.

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BMC Plant Biology

ISSN: 1471-2229

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