Fig. 1

Analyses of OsZIP1 expression under metal stress conditions using qRT-PCR and Western blot. Two week-old rice plants grew in the nutrient solutions with 1000 and 10,000 μM Zn, 500 and 5000 μM Mn, 10 and 30 μM Cu, 100 and 1000 μM Fe and 0–160 μM Cd for 3 h. For Western blot analysis, seedlings were exposed to the metals at the indicated concentrations for 4 d. a Response of OsZIP1 transcription to excess Zn, Mn, Cu and Fe. b Response of OsZIP1 transcription to Cd. c-e Western blotting analysis of OsZIP1 expression in roots under excess Zn and Cu and Cd stress. Vertical bars represent standard deviation. Asterisks indicate that the mean values of three replicates are significantly different between the treatments and control (p < 0.05)