Fig. 5

Zn, Cu and Cd concentrations in shoots and roots of WT, oszip1 mutant, RNAi lines and OX lines under excess metal stress. Two week-old rice seedlings were grown in nutrient solutions supplemented with 1000 μM Zn or 20 μM Cu for 10 d or supplemented with 80 μM Cd for 4 d. 0.4 μM Zn and 0.2 μM Cu were used as the controls. Zn (a-c) and Cu (d-f) concentrations in WT and oszip1 mutants, RNAi lines and OXs lines under normal conditions. Zn (g-i) and Cu (j-l) concentrations in WT, oszip1 mutant, RNAi lines and OX lines under excess Zn and Cu stress. m-o Cd concentrations in WT, oszip1 mutant, RNAi lines and OX lines exposed to 80 μM. Vertical bars represent standard deviation. Asterisks indicate that the mean values are significantly different between the oszip1 mutant/ RNAi lines/OX lines and wild-type (p < 0.05)