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Fig. 1 | BMC Plant Biology

Fig. 1

From: Demonstration of highly efficient dual gRNA CRISPR/Cas9 editing of the homeologous GmFAD2–1A and GmFAD2–1B genes to yield a high oleic, low linoleic and α-linolenic acid phenotype in soybean

Fig. 1

Diagram of dual gRNA CRISPR/Cas9 vector, target sequences and target locations. a CRISPS/Cas9 vector for soybean transformation with pFGC5941 backbone, bar gene as selection marker, Cas9 sequence (hCAS9) and two sgRNAs. Cas9 and gRNA expression are driven by the 35S promoter and Arabidopsis U6 promoter (pU6). MAS, Manopine promoter. Nos, Nopaline Synthase terminator; MAS-Ter, Manopine Synthase terminator; FLAG, 3x FLAG sequences. b sgRNA sequences; c Location of dual target sites in GmFAD2–1A. d Location of dual target sites in GmFAD2–1B. Gene-specific primers used for PCR genotyping are indicated by arrows

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BMC Plant Biology

ISSN: 1471-2229

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