Skip to main content
Fig. 4 | BMC Plant Biology

Fig. 4

From: Demonstration of highly efficient dual gRNA CRISPR/Cas9 editing of the homeologous GmFAD2–1A and GmFAD2–1B genes to yield a high oleic, low linoleic and α-linolenic acid phenotype in soybean

Fig. 4

CRISPR/Cas9-induced mutations in GmFAD2–1B in T0 transgenic plants. a Expected deletions due to excision of DNA sequences between the PAM sites. b Partial deletions in transgenic line ND1–55. c Small deletions/insertion in target 1 and/or target 2. The number of PCR amplicons giving mutant sequences out of the total amplicons sequenced (Clones) and the indels (Δ) detected in target 1 (left values) or target 2 (right values) for each of the ten T0 events are indicated. Inherited Indels in T1 and/or T2 progenies are marked by ** and chimeric Indels are marked by N. -, deleted nucleotides; +, inserted nucleotides; 0, no deletion or insertion; a, b, different alleles detected in each T0 event

Back to article page