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Fig. 2 | BMC Plant Biology

Fig. 2

From: Expression of AtLEC2 and AtIPTs promotes embryogenic callus formation and shoot regeneration in tobacco

Fig. 2

Seedlings of wild type and different transgenic lines grown on 20 μM DEX containing medium. a-e The phenotype of 5 DAG seedlings under 20 μM DEX induction. Scale bars = 2 mm. f-j The upper part shows the 12 DAG seedlings under 20 μM DEX induction. Scale bars = 2 mm; the lower part shows the enlarged images. Scale bars = 6 mm. k-o The upper part shows the 20 DAG seedlings under 20 μM DEX induction. Scale bars = 2 mm; the lower part shows the enlarged images. Scale bars = 6 mm. p Measurement of the hypocotyl (Left) and primary root (Right) lengths of seedlings. Values are mean ± standard errors form 20 replicates. Single, double, and three asterisks indicate statistically significant differences from the wild type (at the corresponding DAG: 5 DAG, 12 DAG, and 20 DAG, respectively) (*P < 0.05, **P < 0.01, and ***P < 0.001, Student’s t-test). The pictures were captured using line 5 of AtIPT3-OE AtLEC2-GR transgenic lines, line 9 of AtIPT7-OE AtLEC2-GR transgenic lines and line 8 of AtIPT9-OE AtLEC2-GR transgenic lines. White arrows point to embryogenic callus

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