Fig. 7

MdMYB30 modulates cuticular wax-related resistance to B. dothidea in apple fruit. a Expression levels of MdMYB30 around the injection sites according to qRT-PCR assays. MdMYB30-pIR was the MdMYB30 transient overexpression vector, while the MdMYB30-TRV was the MdMYB30 transient antisense vector. Empty pIR and TRV vectors were used as controls. b Expression of wax-synthesis-related genes around the injection sites. c Scanning electron microscopic images of cuticular wax crystal patterns of infected apple fruit peel around the injection sites (scale bars: 10 μm). The numbers of wax crystals around the MdMYB30-TRV/MdMYB30-pIR injection sites were less/more than the TRV/pIR empty vector counterparts (arrows point to the epicuticular wax crystals). d Phenotypes of TRV, MdMYB30-TRV, pIR, and MdMYB30-pIR infected with B. dothidea for 4 days after the injection. (scale bars: 1 cm) e Fungal plaque areas of apple after being infected with B. dothidea for 4 days. f Expression of apple disease resistance-related genes with the qRT-PCR assay around the injection sites after infection with B. dothidea for 4 days