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Table 4 Preference of Spodoptora littoralis larvae towards different cotton lines and different treatments. Third instars were given the choice between leaf discs from two different plants (Plant A vs Plant B). The leaf discs were either taken from conventional cotton (Coker 312) or two ULGCS cotton lines (RNAi 1: 66-49B and RNAi 2: 66–274). Additionally, plants were either untreated, damaged by larvae or treated with jasmonic acid. After 24 h the consumed leaf surface was measured. Preference indices were calculated as: (leaf area consumed Plant A – leaf area consumed Plant B)/total leaf area consumed. Three larvae received leaf discs from one plant pair and data were pooled for analyses, twenty plant pairs were tested per comparison (n = 20). Un = untreated; Spod = damage by S. littoralis larvae; JA = treated with jasmonic acid. The consumed leaf areas for Plant A and Plant B were compared using one-sided Wilcoxon signed-rank tests

From: Constitutive and induced insect resistance in RNAi-mediated ultra-low gossypol cottonseed cotton

Plant A

Plant B

Leaf area Plant A (cm2 ± SE)

Leaf area Plant B (cm2 ± SE)

Preference for Plant A (± SE)

Statistics

Coker Un

Coker Spod

1.216 ± 0.161

0.108 ± 0.030

0.76 ± 0.062

V = 209; p < 0.001

Coker Un

Coker JA

0.998 ± 0.151

0.334 ± 0.062

0.39 ± 0.119

V = 181; p = 0.003

RNAi 1 Un

RNAi 1 Spod

1.082 ± 0.137

0.324 ± 0.077

0.57 ± 0.079

V = 204; p = 0.001

RNAi 1 Un

RNAi 1 JA

1.191 ± 0.198

0.461 ± 0.098

0.39 ± 0.105

V = 186; p = 0.003

Coker Un

RNAi 1 Un

0.628 ± 0.086

0.709 ± 0.145

0.12 ± 0.135

V = 128; p = 0.737

Coker Spod

RNAi 1 Spod

0.549 ± 0.156

0.652 ± 0.106

− 0.28 ± 0.145

V = 59; p = 0.926

Coker JA

RNAi 1 JA

0.391 ± 0.102

0.760 ± 0.126

− 0.30 ± 0.124

V = 43; p = 0.120

RNAi 2 Un

RNAi 2 Spod

1.291 ± 0.167

0.169 ± 0.053

0.69 ± 0.090

V = 201; p < 0.001

RNAi 2 Un

RNAi 2 JA

1.017 ± 0.145

0.291 ± 0.068

0.52 ± 0.100

V = 195; p < 0.001

Coker Un

RNAi 2 Un

0.706 ± 0.130

0.836 ± 0.106

− 0.18 ± 0.096

V = 50; p = 0.120

Coker Spod

RNAi 2 Spod

0.415 ± 0.088

0.612 ± 0.108

− 0.18 ± 0.129

V = 72; p = 0.461

Coker JA

RNAi 2 JA

0.664 ± 0.123

0.598 ± 0.095

0.02 ± 0.104

V = 108; p = 0.926