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Fig. 8 | BMC Plant Biology

Fig. 8

From: Comparative secretome analysis of different smut fungi and identification of plant cell death-inducing secreted proteins from Tilletia horrida

Fig. 8

Expression profiles of two putative effector genes during T. horrida infection of the kernel smut–resistant and -susceptible rice male sterile lines. The T. horrida–inoculated panicles of the kernel smut–resistant cultivar Jiangcheng 3A (resistant rice male sterile line, R) and susceptible cultivar 9311A (susceptible rice male sterile line, S) were collected at 0, 8, 12, 24, 48, and 72 h post-inoculation for gene expression analyses using quantitative real time reverse transcription-polymerase chain reaction (qRT-PCR) assay. The UBQ gene was used to normalize the data, which are presented as normalized relative quantities scaled to the control. Error bars indicated the standard deviation of five independent replicates. For statistical analysis, we used analysis of variance with the R-package “ggpubr”, and significance was set at P < 0.05. *: P < 0.05, **: P < 0.01, ***: P < 0.001

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