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Fig. 4 | BMC Plant Biology

Fig. 4

From: GSTU43 gene involved in ALA-regulated redox homeostasis, to maintain coordinated chlorophyll synthesis of tomato at low temperature

Fig. 4

ALA induced changes of glutathione S-transferase (GSTU43) gene and protein expression and GST activity in tomato leaves under low temperature within 48 h. The tomato leaves treated with distilled water or 25 mg·L− 1 ALA then exposed to normal condition (control and ALA) or low temperature (LT and LTA) 12 h later. The expression of GSTU43 (the gene transcription levels in control plants at 0 h was normalized as 1) (a) and GST activity (b) were carried out when the low temperature started. The tomato leaves were pretreated with 100 μM Gabaculine (GAB, inhibition of ALA synthesis that inactivates glutamate-1-semialdehyde aminotransferase). After 8 h, the leaves were sprayed with distilled water or 25 mg·L− 1 ALA. Twelve hours later, the plants were exposed to low temperatures. After 24 h low temperatures, the GSTU43 gene expression (the gene transcription levels in control plants at 24 h was normalized as 1) (c) and GST activity (d), and protein levels (e) were measured. Data are expressed as the mean ± standard error of three independent biological replicates. The experiments were repeated for three times. Different letters above the bars indicate a significant difference determined by one-way ANOVA with Tukey’s test (P < 0.05)

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