Fig. 2From: Proximity-dependent biotinylation screening identifies NbHYPK as a novel interacting partner of ATG8 in plantsImmunoblot analysis of BioID fusion proteins and total biotinylated proteins. a Western blots were carried out using anti-ATG8, or anti-mCherry antisera to ascertain fusion protein expression. b Streptavidin-HRP blot of crude protein lysate. c Strep-HRP blot after Dynabeads™ purification. Lower panels show Ponceau S stained blot membranes to confirm equal loading of total proteinsBack to article page