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Fig. 3 | BMC Plant Biology

Fig. 3

From: The differential response of cold-experienced Arabidopsis thaliana to larval herbivory benefits an insect generalist, but not a specialist

Fig. 3

Regulation of gene expression in response to herbivory or artificial wounding in Arabidopsis thaliana leaves compared to untreated control leaves. Plants were exposed to feeding by P. brassicae larvae (Tp), M. brassicae larvae (TM), artificial wounding (TW) or were left untreated (C2). Here, the TP, TM and TW treatments were adjusted in such a way that we obtained comparable extent of leaf damage (about 60 mm2 per plant; see Additional file 1: Figure S4). Plant material for microarray analysis was collected 2 days later. N = 3 biological replicates of each sample type. a Principle component analysis (PCA) of transcriptomic patterns of individual samples collected for microarray analysis. Samples originated from untreated control plants (C2, purple), feeding-damaged plants by either P. brassicae (TP, red) or M. brassicae (TM, blue) or artificially wounded plants (TW, green). The first two principal components, which explain most of the changes, are depicted (explained variances are shown at the axes). Ellipses indicate the 95% confidence interval. b The Venn diagram shows the number of genes, which were upregulated (upwards pointing arrows) or downregulated (downwards pointing arrows) in Tp, TM and TW samples compared to C2 samples. c Heatmap depicting genes, which show opposed regulation in at least two treatments. Yellow = upregulated, blue = downregulated, grey = not regulated (log2 fold changes). d Gene Ontology terms associated with commonly or uniquely up- and downregulated genes

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