Table 1 Protein information, conserved amino acid residues, transmembrane domains (TMDs), and their predicated subcellular localization of Cucumis sativus aquaporins
Name | Gene ID | aLength | bMW | cpI | dGRAVY | eTM | Ar/R selectivity filter | NPA motifs | Froger’s positions | fLoc | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
H2 | H5 | LE1 | LE2 | LB | LE | P1 | P2 | P3 | P4 | p5 | ||||||||
CsPIP1;1 | Csa5M199270.1 | 292 | 31.43 | 7.67 | 0.31 | 6 | F | H | T | R | NPA | NPA | Q | S | A | F | W | plas |
CsPIP1;2a | Csa5M198770.1 | 292 | 31.37 | 7.68 | 0.34 | 6 | F | H | T | R | NPA | NPA | Q | S | A | F | W | plas |
CsPIP1;2b | Csa5M198770.2 | 217 | 23.20 | 7.62 | 0.21 | 4 | F | / | / | / | NPA | / | Q | / | / | / | / | plas |
CsPIP1;3 | Csa5M153020.1 | 286 | 30.76 | 9.23 | 0.36 | 6 | F | H | T | R | NPA | NPA | Q | S | A | F | W | plas |
CsPIP1;4 | Csa5M199280.1 | 292 | 31.40 | 7.67 | 0.29 | 5 | F | H | T | R | NPA | NPA | Q | S | A | F | W | plas |
CsPIP1;5 | Csa6M445090.1 | 191 | 20.26 | 9.51 | 0.47 | 4 | / | N | A | R | NPA | NPA | K | S | A | F | W | plas |
CsPIP1;6 | Csa3M739030.1 | 290 | 31.25 | 9.10 | 0.49 | 6 | F | H | T | R | NPA | NPA | Q | S | A | F | W | plas |
CsPIP2;1a | Csa6M445130.1 | 284 | 29.92 | 7.04 | 0.51 | 6 | F | H | T | R | NPA | NPA | Q | S | A | F | W | plas |
CsPIP2;1b | Csa6M445130.2 | 208 | 21.92 | 6.18 | 0.45 | 4 | F | S | / | / | NPA | / | Q | / | / | / | / | plas |
CsPIP2;2 | Csa6M445120.1 | 284 | 30.24 | 8.78 | 0.56 | 6 | F | H | T | R | NPA | NPA | Q | S | A | F | W | plas |
CsPIP2;3a | Csa6M445140.1 | 284 | 29.98 | 7.64 | 0.56 | 7 | F | A | F | T | NPA | HLA | Q | I | G | F | N | plas |
CsPIP2;3b | Csa6M445140.2 | 208 | 21.93 | 6.26 | 0.56 | 4 | F | S | / | / | NPA | / | Q | / | / | / | / | plas |
CsPIP2;4a | Csa6M140850.1 | 283 | 30.24 | 7.63 | 0.47 | 6 | F | H | T | R | NPA | NPA | Q | S | A | F | W | plas |
CsPIP2;4b | Csa6M140850.2 | 252 | 26.85 | 6.06 | 0.47 | 5 | F | H | T | R | NPA | NPA | Q | S | A | S | L | plas |
CsPIP2;5 | Csa6M405320.1 | 276 | 29.37 | 9.36 | 0.53 | 6 | F | H | T | R | NPA | NPA | Q | S | A | F | W | plas |
CsPIP2;6a | Csa6M445150.1 | 279 | 29.72 | 8.97 | 0.62 | 6 | F | H | T | R | NPA | NPA | Q | S | A | F | W | plas |
CsPIP2;6b | Csa6M445150.2 | 197 | 20.96 | 9.75 | 0.69 | 5 | F | H | T | R | NPA | NPA | Q | S | A | F | W | plas |
CsPIP2;7 | Csa5M623360.1 | 287 | 30.52 | 9.11 | 0.41 | 6 | F | H | T | R | NPA | NPA | Q | S | A | F | W | plas |
CsPIP2;8 | Csa7M014450.1 | 280 | 29.85 | 9.24 | 0.45 | 6 | F | H | T | R | NPA | NPA | M | S | A | F | W | plas |
CsTIP1;1 | Csa6M448110.1 | 250 | 25.72 | 5.64 | 0.78 | 7 | H | I | A | V | NPA | NPA | F | A | S | Y | W | vacu |
CsTIP1;2 | Csa3M743400.1 | 253 | 26.29 | 6.03 | 0.93 | 7 | H | I | A | V | NPA | NPA | F | A | A | Y | W | vacu |
CsTIP1;3 | Csa5M505790.1 | 254 | 26.53 | 5.30 | 0.71 | 6 | H | I | A | V | NPA | NPA | F | T | A | Y | W | vacu |
CsTIP2;1 | Csa5M162580.1 | 250 | 25.09 | 5.39 | 0.99 | 6 | H | I | G | R | NPA | NPA | F | S | A | Y | W | plas |
CsTIP2;2 | Csa7M447100.1 | 248 | 25.44 | 5.66 | 0.87 | 6 | H | I | G | R | NPA | NPA | Y | S | A | Y | W | vacu |
CsTIP3;1 | Csa1M043290.1 | 289 | 30.66 | 7.17 | 0.46 | 5 | H | I | A | R | NPA | NPA | L | A | S | Y | W | vacu |
CsTIP4;1 | Csa2M374630.1 | 247 | 25.72 | 6.01 | 0.90 | 7 | H | I | A | R | NPA | NPA | Y | S | A | Y | W | vacu |
CsTIP5;1 | Csa5M168860.1 | 255 | 26.12 | 6.88 | 0.86 | 6 | N | V | G | C | NPA | NPA | A | A | A | Y | W | plas |
CsNIP1;1 | Csa6M520340.1 | 276 | 29.45 | 9.58 | 0.54 | 6 | W | V | A | R | NPA | NPA | F | S | A | Y | I | plas |
CsNIP1;2 | Csa3M345890.1 | 269 | 28.88 | 7.67 | 0.78 | 5 | W | V | A | R | NPA | NPA | F | S | A | Y | M | vacu |
CsNIP2;1 | Csa3M826640.1 | 288 | 30.55 | 9.40 | 0.33 | 6 | G | S | G | R | NPA | NPV | L | T | A | Y | F | vacu/plas |
CsNIP2;2 | Csa3M826650.1 | 261 | 27.62 | 6.05 | 0.45 | 6 | C | S | G | R | NPA | NPA | M | S | A | Y | M | plas |
CsNIP3;1a | Csa5M146200.1 | 249 | 26.01 | 8.86 | 0.65 | 4 | A | I | G | R | NPS | NPV | F | T | A | Y | M | plas |
CsNIP3;1b | Csa5M146200.2 | 221 | 23.51 | 8.50 | 0.74 | 6 | A | I | / | / | NPS | / | F | / | / | F | l | plas |
CsNIP3;2 | Csa5M146190.1 | 298 | 30.80 | 8.63 | 0.50 | 5 | A | I | G | R | NPS | NPV | F | A | A | Y | l | plas |
CsNIP3;3 | Csa4M007030.1 | 304 | 31.52 | 8.26 | 0.43 | 5 | T | V | A | R | NPA | NPV | F | T | A | Y | l | plas |
CsNIP4;1 | Csa3M149960.1 | 268 | 28.45 | 6.88 | 0.68 | 6 | A | V | A | R | NPA | NPA | F | S | A | Y | I | plas/vacu |
CsSIP1;1 | Csa4M192210.1 | 243 | 25.59 | 9.41 | 0.83 | 6 | V | T | P | N | NPT | NPA | F | A | A | Y | W | vacu |
CsSIP2;1 | Csa3M816140.1 | 238 | 25.93 | 10.00 | 0.66 | 5 | F | K | G | S | NPL | NPA | F | V | A | Y | W | vacu |
CsXIP1;1 | Csa2M263850.1 | 319 | 34.47 | 8.27 | 0.66 | 7 | V | I | V | R | SPI | SPA | Y | C | A | F | W | cyto |
/ | Csa6M445100.1 | 106 | 11.57 | 4.51 | 0.62 | 2 | F | / | / | / | / | / | / | / | / | / | / | plas |
/ | Csa7M336420.1 | 99 | 10.66 | 4.83 | 0.72 | 3 | / | / | / | / | / | / | / | / | / | / | / | mito |