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Table 1 Number of alleles per locus and polymorphic information content (PIC) value of genic-SSR markers used for studying allelic variation among blackgram genotypes

From: Transcriptome sequencing, de novo assembly, characterisation of wild accession of blackgram (Vigna mungo var. silvestris) as a rich resource for development of molecular markers and validation of SNPs by high resolution melting (HRM) analysis

Genic-SSR primer code

No. of alleles /locus

Polymorphic information content (PIC)

Putative function

TWSSR-1

2

0.28

Protein ABCI7

TWSSR-4

2

0.21

transcription factor bHLH143-like

TWSSR-8

3

0.76

CBL-interacting serine/threonine-protein kinase 8

TWSSR-9

3

0.62

Vigna angularis var. angularis DNA, chromosome 11

TWSSR-10

5

0.67

transcription factor TCP9

TWSSR-11

2

0.53

U-box domain-containing protein 4

TWSSR-12

4

0.49

Vigna radiata var. radiata uncharacterized LOC106765753

TWSSR-13

2

0.14

sugar carrier protein C

TWSSR-14

3

0.34

Vigna angularis var. angularis DNA, chromosome 1

TWSSR-15

3

0.60

vacuolar sorting receptor

TWSSR-16

4

0.50

Vigna radiata var. radiata uncharacterized LOC106778819

TWSSR-19

3

0.67

alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1

TWSSR-20

4

0.72

transcription factor 25

TWSSR-24

1

0.14

glycerol-3-phosphate transporter 4

TWSSR-31

2

0.58

Lupinus angustifolius cultivar Tanjil chromosome LG-11

TWSSR-34

2

0.59

60S ribosomal protein L29–1

TWSSR-47

3

0.48

hypothetical protein (PHAVU_001G023500g)

TWSSR-48

2

0.50

Human DNA sequence from clone RP11-164 K15 on chromosome 22

TWSSR-57

2

0.48

vesicle-associated membrane protein 722-like

TWSSR-59

3

0.57

Vigna angularis var. angularis DNA, chromosome 4

TWSSR-61

3

0.85

glycine-rich domain-containing protein 1

TWSSR-62

4

0.69

transcription initiation factor IIF subunit beta

TWSSR-66

3

0.52

Vigna radiata var. radiata uncharacterized LOC106765616

TWSSR-68

3

0.79

UPF0496 protein At2g18630-like

TWSSR-72

2

0.46

Vigna angularis uncharacterized LOC108326918

TWSSR-74

4

0.77

ATP-dependent RNA helicase eIF4A

TWSSR-76

1

0.65

activator of 90 kDa heat shock protein ATPase homolog

TWSSR-81

1

0.36

trafficking protein particle complex subunit 6B

TWSSR-82

3

0.69

Vigna angularis var. angularis DNA, chromosome 8

TWSSR-86

3

0.54

serine/threonine-protein kinase-like protein At3g51990

TWSSR-87

3

0.46

histidine kinase 4

Average

3

0.54

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