Table 1 Copy numbers of target genes in transgenic maize through micro-droplet digital PCR
From: Genetic variation assessment of stacked-trait transgenic maize via conventional breeding
Sample | Gene | Total number of micro-droplets | Density (copies/μL) | Copy number |
|---|---|---|---|---|
IE034 | Hmga | 14,406 ± 1001 | 265 ± 18.25 |  |
cry1Ie | 15,414 ± 1181 | 250 ± 40.67 | 0.94 | |
12–5 × IE034 | Hmga | 16,071 ± 810 | 274 ± 5.57 |  |
cry1Ie | 18,776 ± 332 | 128 ± 5.13 | 0.47 | |
cry1Ab | 18,484 ± 289 | 135 ± 3.79 | 0.49 | |
G10-EPSPs | 18,439 ± 730 | 136 ± 3.61 | 0.5 | |
12–5 | Hmga | 17,321 ± 510 | 264 ± 15.40 |  |
cry1Ab | 16,930 ± 1741 | 268 ± 34.95 | 1.02 | |
G10-EPSPs | 17,573 ± 322 | 282 ± 18.82 | 1.07 |