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Fig. 5 | BMC Plant Biology

Fig. 5

From: New perspectives on the plant PARP family: Arabidopsis PARP3 is inactive, and PARP1 exhibits predominant poly (ADP-ribose) polymerase activity in response to DNA damage

Fig. 5

AtPARP3 is incapable of binding to the substrate NAD+. a Immunoblotting with streptavidin/HRP. b Immunoblotting with anti-His antibody. The purified proteins were blotted onto an activated PVDF membrane and then incubated with 25 μM biotinylated NAD+ in the presence of 500 nM broken DNA for 30 min at 25 °C. The NAD+ binding proteins were detected by blotting with streptavidin/HRP. An anti-His antibody was used to visualize the loading amounts of the proteins blotted on the membrane

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