Fig. 3

Expression of soybean PHO1 genes in response to salt stress. a-e The expression of Gm-GsPHO1 orthologous gene pairs in Class I. f-i The expression of Gm-GsPHO1 orthologous gene pairs in Class II. Total RNAs from roots of two-week old seedlings of ZYD6 and SN14 under salt stresses with different concentration of NaCl for 4 h were subjected to qRT-PCR. Expression of each gene in the non-treated conditions (gray column) was used as control. For ease in comparison of Gm-GsPHO1 orthologous gene pairs, the GmPHO1 expression was set as 1 under the untreated conditions. The * means significance at the P < 0.05 level, and the ** represent the significance at the P < 0.01 level