Fig. 5From: Reconstruction of the full-length transcriptome atlas using PacBio Iso-Seq provides insight into the alternative splicing in Gossypium australeSashimi plot and RT-PCR showing transcript isoforms of PB.13680 (MK). a Sashimi plot shows the intron retention level of PB.13680. Peaks in red represent short-read coverage. For each isoform, blocks in blue represent exons, and lines between blocks represent introns. Red triangles indicate the position of primers used for RT-PCR. b RT-PCR validation of the differential intron retention level of PB.13680. cDNA from 5 h, 15 h and 30 h germinating seeds and leaf samples were used for RT-PCR. The gel bands show DNA markers and the RT-PCR products from four tissues/samples. The expected sizes are shown in the right panel. The percent of isoforms relative expression are shown in the below panelBack to article page