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Fig. 1 | BMC Plant Biology

Fig. 1

From: Cyclic nucleotide-gated ion channel 6 mediates thermotolerance in Arabidopsis seedlings by regulating nitric oxide production via cytosolic calcium ions

Fig. 1

Effects of Ca2+ on NO accumulation in Arabidopsis seedlings. (a) Eight-day-old wild-type, cngc6, COM12, and OE8 seedlings grown at 22 °C were exposed to 45 °C (HS) or maintained at 22 °C (Control) for 60 min. The NO levels in the seedlings were then examined by fluorescence microscopy using roots dyed with DAF-FM DA. Bar = 100 μm. (b) Relative DAF-FM fluorescence densities in the roots. The data presented are the means ± standard error (SE) of measurements taken from five independent experiments with at least ten roots for each treatment. *P < 0.05 versus Col (Student’s t-test). (c) Eight-day-old wild-type, cngc6, and OE8 seedlings were exposed to 45 °C (HS) or maintained at 22 °C (Control) for 60 min. The NO levels in the plants were then examined by fluorescence microscopy using roots stained with DAF-FM DA. Bar = 100 μm. (d) The relative DAF-FM fluorescence densities in the roots. The data presented are the means ± SE of measurements taken from five independent experiments with at least ten roots for each treatment. *P < 0.05 versus 0 mM CaCl2 (Student’s t-test)

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