Fig. 6

Effects of CNGC6 via NO on AtHSP17.7 and AtHSP21 expression. (a–d) Ten-day-old seedlings grown at 22 °C were exposed to 37 °C (HS) or maintained at 22 °C (Control) for 2 h. Total protein was then extracted, separated by SDS-PAGE, and analyzed by Western blotting. Tubulin was used as an internal control. Three independent experiments were carried out; the results indicate similar trends in protein accumulation. The numbers below each lane represent the relative intensity of each signal