- Research article
- Open Access
Overexpression of the trehalose-6-phosphate phosphatase family gene AtTPPF improves the drought tolerance of Arabidopsis thaliana
BMC Plant Biology volume 19, Article number: 381 (2019)
Trehalose-6-phosphate phosphatases (TPPs), which are encoded by members of the TPP gene family, can improve the drought tolerance of plants. However, the molecular mechanisms underlying the dynamic regulation of TPP genes during drought stress remain unclear. In this study, we explored the function of an Arabidopsis TPP gene by conducting comparative analyses of a loss-of-function mutant and overexpression lines.
The loss-of-function mutation of Arabidopsis thaliana TPPF, a member of the TPP gene family, resulted in a drought-sensitive phenotype, while a line overexpressing TPPF showed significantly increased drought tolerance and trehalose accumulation. Compared with wild-type plants, tppf1 mutants accumulated more H2O2 under drought, while AtTPPF-overexpressing plants accumulated less H2O2 under drought. Overexpression of AtTPPF led to increased contents of trehalose, sucrose, and total soluble sugars under drought conditions; these compounds may play a role in scavenging reactive oxygen species. Yeast one-hybrid and luciferase activity assays revealed that DREB1A could bind to the DRE/CRT element within the AtTPPF promoter and activate the expression of AtTPPF. A transcriptome analysis of the TPPF-overexpressing plants revealed that the expression levels of drought-repressed genes involved in electron transport activity and cell wall modification were upregulated, while those of stress-related transcription factors related to water deprivation were downregulated. These results indicate that, as well as its involvement in regulating trehalose and soluble sugars, AtTPPF is involved in regulating the transcription of stress-responsive genes.
AtTPPF functions in regulating levels of trehalose, reactive oxygen species, and sucrose levels during drought stress, and the expression of AtTPPF is activated by DREB1A in Arabidopsis. These findings shed light on the molecular mechanism by which AtTPPF regulates the response to drought stress.
Trehalose is a non-reducing disaccharide comprising two Glc units linked in an α, α-1, 1-glucoside configuration. This compound exists in plants, fungi, bacteria, and invertebrate animals . In addition to its function as a carbon source, it also acts as a protective compound under adverse conditions, such as dehydration, high salinity, hypoxia, and nutrient starvation [1, 2]. For example, trehalose functions as a protectant to stabilize membranes and proteins in certain resurrection plants such as Myrothamnus flabellifolius and Sporobolus spp., allowing them to survive during dehydration–rehydration cycles [3, 4].
Five trehalose synthesis pathways exist in prokaryotes, but only the trehalose-6-phosphate (T6P) synthase (TPS)/T6P phosphatase (TPP) pathway exists in eukaryotes . In this pathway, T6P is first synthesized from UDP-Glc and Glc-6-phosphate by TPS and then dephosphorylated to trehalose by TPP . As an important phosphorylated intermediate in the trehalose synthetic pathway, T6P is an essential sugar-signaling metabolite that regulates plant metabolism and other biological processes [5,6,7,8]. The molecular mechanisms underlying the accumulation of trehalose and T6P, with the latter contributing more to increased drought tolerance, are not yet understood. However, the accumulation of soluble sugars such as sucrose and trehalose may be a protective mechanism under oxidative stress conditions [9,10,11]. Because of the relatively low abundance of trehalose, it may have little effect on osmotic regulation unless its distribution within cells is specifically compartmentalized . In Arabidopsis, T6P is a specific signaling molecule that senses the sucrose concentration [13, 14]. Because T6P exists at a concentration three orders of magnitude lower than that of sucrose, it is possible that a slight change in the T6P level may be accompanied by dramatic changes in the sucrose concentration [13, 14]. Therefore, changes in T6P levels may contribute more than changes in other sugars to increasing stress tolerance.
The constitutive heterologous overexpression of trehalose biosynthetic genes is a valuable tool for improving the stress tolerance of plants. Thus, TPS and TPP genes have received considerable attention because of their important roles in trehalose biosynthesis [15, 16]. The genome of Arabidopsis thaliana contains 11 TPSs (AtTPS1–11) and 10 TPPs (AtTPPA–J) . Of them, AtTPS1, − 2 and − 4 -exhibit TPS enzymatic activity and all of the TPP proteins exhibit TPP enzymatic activity when heterologously expressed in yeast [15,16,17]. The TPSs and TPPs function as important growth regulators during responses to adverse conditions and in the formation of crop yield. The overexpression of AtTPS1 conferred dehydration tolerance in Arabidopsis, accompanied by small increases in trehalose and T6P levels . The wheat TPP gene TPP-6AL1 was found to be associated with 1000-grain weight and grain yield . From a practical standpoint, the overexpression of rice TPP1 in maize under the control of the flower-specific promoter MADS6 improved yield under varying degrees of drought . Much of the research on trehalose biosynthetic genes has focused on their role of trehalose in stress responses and their function in increasing crop production. The underlying mechanism of TPP regulation and the dynamic regulation of related genes under drought conditions are still unclear.
The 10 TPP genes in Arabidopsis exhibit diverse spatiotemporal expression patterns, indicating that they may have distinct functions . AtTPPD is preferentially expressed in the root cap, and the overexpression of AtTPPD can improve salt tolerance, indicating that this gene plays a role in the response to saline conditions . AtTPPA and AtTPPG tend to be expressed in the protoderm and may play redundant roles during the differentiation of root epidermal cells . AtTPPB is highly expressed in young leaves and functions in increasing the number of leaf cells . AtTPPG has a particular expression profile in stomatal cells and is involved in the regulation of stomatal closure, with tppg mutants exhibiting resistance to abscisic acid (ABA)-mediated stomatal closure . Interestingly, AtTPPF is induced specifically under dehydration conditions. Additionally, compared with wild-type (WT) plants, AtTPPF-overexpressing plants were found to be slightly more sensitive to ABA treatments, as assessed by a stomatal movement assay [16, 22]. This suggests that AtTPPF plays an important role in regulating stomatal movement and may be involved in the regulation of drought responses. Although rice (Oryza sativa) TPP1 was shown to improve tolerance to dehydration and improve grain yields in maize , the molecular mechanisms by which TPP regulates drought responses remain largely unknown.
Here, we provide evidence that the loss-of-function mutant of AtTPPF results in a drought-sensitive phenotype and that the elevated expression of AtTPPF can increase drought tolerance. We discovered that DREB1A targets AtTPPF and mediates drought stress signaling by directly binding the DRE/CRT motif in the AtTPPF promoter, thereby activating its transcription. A global transcriptional analysis revealed that AtTPPF functions in regulating the transcription of genes involved in stress responses.
tppf1 mutant is drought sensitive
A T-DNA insertional mutation in the promoter of Arabidopsis AtTPPF (At4g12430) in the Columbia-0 background was annotated as tppf1 (SALK_087220) (Fig. 1a). We identified a homozygous tppf1 line by PCR amplification using allele-specific primers (Fig. 1b; Additional file 5: Table S1). An RT-PCR analysis confirmed that there were At4g12430 transcripts present in the WT but not in the tppf1 mutant (Fig. 1c), suggesting that the mutation caused a loss of AtTPPF function. To investigate the genetic nature of tppf1, the mutant was back-crossed with WT Columbia-0. A phenotypic examination of the F2 progeny revealed a segregation ratio of 143 resistant: 45 sensitive plants (χ2 [3:1] = 0.258 < χ20.05 = 3.84; p > 0.05), indicating that the tppf1 mutation was inherited as a single recessive trait.
A phylogenetic analysis revealed that AtTPPF is highly similar to OsTPP1 (Additional file 1: Figure S1), which increased drought tolerance when overexpressed in maize . We then analyzed the drought tolerance of the tppf1 mutant. Under a 28-d continuous drought treatment, the tppf1 plants were more sensitive to drought stress than were WT plants, while the phenotypes of the tppf1 plants were similar to those of WT plants under well-watered conditions (Fig. 1d). At 2 d after re-watering, the survival rate of the tppf1 plants was significantly lower than that of WT plants (Fig. 1e). We evaluated the rate of water loss from detached leaves. Although the detached leaves of tppf1 lost water slightly faster than did WT leaves, there was no statistical difference between their water loss rates (Fig. 1f).
AtTPPF overexpression increases drought tolerance
To assess the function of AtTPPF under drought stress, we cloned the AtTPPF cDNA and generated an overexpression construct driven by the CaMV 35S promoter (Fig. 2a). This construct was then transformed into WT plants. Transgenic T3 homozygous lines in which AtTPPF expression levels were greater than that of WT plants were obtained. Three lines with different expression levels (OE6, OE5, and OE9) were selected for further analyses (Fig. 2b). The three lines displayed varying degrees of drought resistance, proportional to their transcript levels of AtTPPF (Fig. 2c). For example, OE9 plants showed the highest AtTPPF expression levels, and grew vigorously after 26 d of drought. The AtTPPF expression levels were relatively lower in OE5 plants, which grew less vigorously than the OE9 plants. The WT and OE6 plants displayed severe wilting symptoms under drought stress (Fig. 2c). The OE5 and OE9 plants had significantly greater survival rates (45.7 and 82.7%, respectively) than that of WT plants (28.4%) under drought stress (Fig. 2d). Thus, overexpression of AtTPPF increased the drought tolerance of plants, and the level of improvement was proportional to the level of AtTPPF gene expression. Because the overexpression of AtTPPF increased drought tolerance and the loss of function of AtTPPF compromised drought-stress tolerance, we concluded that AtTPPF positively regulates drought tolerance in Arabidopsis.
To confirm the function of AtTPPF in the drought response, we performed a complementation experiment with the tppf1 mutant using the same construct as described above. The CaMV 35S overexpression construct was transformed into the tppf1 mutant. Eight T2 transgenic lines were obtained, and complemented lines with AtTPPF expression levels similar to that of WT were selected for further analyses. The phenotypes of the complemented lines were similar to those of WT plants under drought conditions (Additional file 2: Figure S2). These results confirmed that the loss-of-function mutation of AtTPPF conferred drought sensitivity in Arabidopsis.
DREB1A binds to drought-responsive DRE/CRT element in TPPF promoter
Searches of the promoter region of AtTPPF (the 2-kb region upstream from the transcriptional initiation site) revealed a DRE/CRT cis-acting element, “ACCGAC,” and three cis-acting ABA-responsive elements (ABREs). Because DRE/CRT elements can interact specifically with DREB/CBF transcription factors, we analyzed coexpression models of AtTPPF and the DREB-coding genes DREB1A, DREB2A, and DREB1B, using the AtGenExpress Visualization Tool. The expression of AtTPPF was essentially consistent with that of DREB1A (Additional file 3: Figure S3). To confirm this coexpression pattern, we used RT-qPCR to quantify the transcript levels of AtTPPF and DREB1A under drought conditions. Both genes were induced by drought, and their expression patterns were essentially similar (Fig. 3a and b). These data suggest that DREB1A may activate the expression of AtTPPF by binding to the DRE/CRT motif.
To determine whether DREB1A recognizes the AtTPPF promoter in vivo, we performed a yeast one-hybrid assay to determine whether DREB1A can directly bind to the DRE/CRT “ACCGAC” motif in the AtTPPF promoter region. The full-length 2-kb promoter of AtTPPF (including DRE/CRT and three ABRE motifs) was cloned into a bait construct. Interestingly, a blue colony without any prey proteins was identified, indicating that the full-length fragment was autonomously activated (Additional file 4: Figure S4). When a 539-bp P1 fragment containing the DRE/CRT motif and the ABRE motif was cloned into the bait construct (Fig. 3c), blue colonies were observed only in the presence of DREB1A prey (Fig. 3d), indicating that DREB1A could recognize and bind to the “ACCGAC” motif in the AtTPPF promoter. However, when AREB1 (binding to the ABRE motif) was added as prey, no blue colonies were obtained (Additional file 4: Figure S4), indicating that there was no interaction between the P1-containing ABRE motif and AREB1. These results indicate that the AtTPPF promoter is recognized specifically by DREB1A.
DREB1A encodes a member of the DREB subfamily of the ERF/AP2 family of transcriptional activators . To determine whether DREB1A can activate the expression of AtTPPF, we performed a dual luciferase (LUC) assay to analyze reporter gene expression. The P1 fragment was introduced into the pGreen II 0800-LUC vector to generate a reporter construct, and the cDNA of DREB1A was inserted into 62-SK to generate an effector construct. Tobacco (Nicotiana benthamiana) leaves were cotransformed with both the effector and reporter constructs and then relative LUC activity was determined. As expected, LUC activity was higher in the presence of both the effector and reporter constructs, including both 0800 + 35S-DREB1A and P1:luc + 62-SK (Fig. 3d, e), than in the presence of the negative control. This result implies that DREB1A can act as a transcriptional activator of AtTPPF.
DREB1A positively modulates AtTPPF expression
To further confirm the direct regulation of AtTPPF by DREB1A, we acquired related DREB1A lines. These lines included one T-DNA insertional mutant (dreb1a) in the untranslated region of DREB1A (At4g25480) in the WT Columbia-0 background (SALK_018603) (Fig. 4a); and one overexpression line of DREB1A in the Wassilewskija (WS) background (CS69502). The homozygous dreb1a line was identified by PCR amplification using allele-specific primers (Fig. 4b). An RT-PCR analysis confirmed that no DREB1A transcripts were present in the dreb1a mutant compared with WT (Fig. 4c). Compared with WS plants, DREB1A-overexpression plants (DREB1A-OX) showed higher transcript levels of DREB1A (Fig. 4d).
Previously, we confirmed that DREB1A could activate AtTPPF transcription (Fig. 3). Consequently, we performed expression assays of AtTPPF in both dreb1a mutants and DREB1A-OX plants. As expected, the transcript abundance of AtTPPF was much lower in the dreb1a plants than in WT plants under both well-watered control and drought conditions. Thus, the loss of DREB1A led to reduced AtTPPF expression. In contrast, compared with WS plants, the DREB1A-OX plants showed increased transcript levels of AtTPPF under both control and drought conditions. Thus, we concluded that DREB1A plays a positive role in AtTPPF expression.
AtTPPF regulates trehalose and H2O2 levels in response to drought stress
AtTPPF encodes a protein in the haloacid dehalogenase-like hydrolase superfamily that is involved in trehalose biosynthesis. Thus, we monitored changes in the trehalose content before and after the drought treatment. The trehalose contents did not increase in the WT and tppf1 mutants, and but accumulated to higher levels in OE9 plants than in WT or tppf1 mutants under drought conditions (Fig. 5a). These results indicate that AtTPPF overexpression leads to the accumulation of trehalose in response to drought stress.
AtTPPF is expressed in the shoot apical meristems (SAM) , so we measured the transcript levels of AtTPPF in the SAM and the REST (the rest part of whole seedling apart from the SAM). As expected, there was a significant difference in AtTPPF transcript abundance between the SAM and the REST under both control and drought conditions (Fig. 5b), indicating that AtTPPF is preferentially expressed in the SAM. The distinct difference in transcript abundance levels occurred specifically under drought conditions, suggesting that AtTPPF plays a key role in the response to drought stress in the SAM. We did not find an appreciable increase in the trehalose levels in whole seedlings of WT and tppf1 under drought stress. Therefore, we measured the trehalose content in the SAM to detect subtle changes. We measured the trehalose contents in the SAM and the REST under control and drought conditions, and found limited differences in trehalose levels in the SAM between the two conditions (Fig. 5c). Although trehalose accumulated in the OE9 plants under drought conditions (Fig. 5a), the increase was relatively small. Thus, this may not be the only explanation for the elevated drought tolerance of AtTPPF-overexpressing plants. Low trehalose levels are not sufficient to control osmoregulation [3, 24]. Therefore, other factors may be responsible for the improved drought tolerance conferred by AtTPPF overexpression.
In plants, H2O2 is a major reactive oxygen species (ROS) that functions as an important messenger to relay drought stress signals . Because AtTPPF was expressed at relatively high levels in the SAM of young seedlings (Fig. 5b), we measured H2O2 levels using DAB staining to verify the possible functions of AtTPPF in scavenging ROS in the SAM. Compared with WT plants, the OE9 plants accumulated less H2O2, especially in new leaves, after 24 h of drought treatment. In contrast, the tppf1 mutants accumulated more H2O2 under the same conditions (Fig. 5d). Even though there was no difference in trehalose levels between the tppf1 mutant and WT plants, the loss of function of AtTPPF caused a partial failure of ROS scavenging. These results indicate that AtTPPF may play roles in antioxidant systems to protect plants under drought conditions. Soluble sugars are known to be involved in redox-balancing in plants , so we determined the total soluble sugars contents. As shown in Fig. 5e, compared with the WT, OE9 plants accumulated more soluble sugars, while tppf1 mutants accumulated less soluble sugars, under drought conditions. In plants, T6P plays a significant role in regulating metabolism and development [5, 8]. The overexpression of TPP1 in maize is associated with changes in T6P and sucrose levels . As shown in Fig. 5f, sucrose accumulated to higher levels in OE9 plants than WT plants under control and drought conditions, but did not accumulate in tppf1 mutants. Therefore, AtTPPF expression could lead to increased sucrose levels.
Transcriptome analysis of WT and AtTPPF OE plants under drought conditions
To further evaluate the role of AtTPPF in modulating plant responses to drought stress on a broader scale, we analyzed the transcriptomes of WT and OE9 plants. Total RNAs from 2-week-old seedlings under well-watered and drought conditions were isolated and subjected to RNA-seq, with three biological replicates. A total of 3 GB of clean data was obtained, which was mapped to the gene models in the TAIR 10 assembly . The fragments per kilobase of transcript per million mapped reads (FPKM) values of each gene were calculated for the WT and OE9 plants. By using pairwise comparisons between the WT and OE9 plants, we defined differentially expressed genes (DEGs) as those whose FPKM values changed by at least two-fold (p value < 0.05). A comparative analysis was performed to compare the WT and OE9 plants under well-watered and drought conditions. Among the DEGs (OE9 vs. WT), 440 were upregulated under drought conditions and 475 were downregulated. We expected that some of these genes would contribute to the improved drought tolerance of the AtTPPF-overexpressing plants (Fig. 6a). Interestingly, 318 (72%) of the 440 upregulated genes in OE9 were repressed in WT plants under drought conditions (Fig. 6a), while 207 (44%) of the 475 downregulated genes in OE9 were induced in WT plants under drought conditions (Fig. 6a). A possible explanation for these results is that the elevated trehalose levels in the SAM may relieve severe effects of drought by modulating the expression of drought-responsive genes.
To test this idea, we performed a gene functional annotation of the 318 upregulated (ERG-U) and 207 downregulated (DIG-D) genes in the OE9 plants (Additional file 7: Table S3 and Additional file 8: Table S4). A gene ontology (GO) analysis revealed that the 318 and 207 genes were involved in various biological and molecular processes (Fig. 6b), especially in responses to stimuli and metabolic processes. The functional annotation analyses suggested that a large portion of these genes encoded transcription factors and enzymes (Fig. 6b). Drought induces plant stomatal closure and reduces photosynthesis . Among the 318 ERG-Us, three were associated with electron transport activity: ENH1, encoding a rubredoxin family protein involved in metal ion binding; CYP81D1, encoding a member of the CYP81D family of cytochrome p450s; and CYP71B13, encoding a putative cytochrome P450. Some genes were involved in carbohydrate metabolism, such as SFP2, which encodes a sugar-porter family protein that participates in sugar transport, as well as SUC1 and SUC5, encoding members of the sucrose-proton symporter family that are involved in sucrose metabolism and transport. The increased expression levels of these genes in OE9 plants may enhance photosynthesis and maintain the carbohydrate balance under drought conditions. In addition, a cell wall biogenesis gene, XTR8, was identified. The product of this gene is involved in cell wall modification and improves cell wall stiffness, further affecting changes in ion leakage . The ERG-Us also included FSD3, encoding a type of peroxidase; FSD3 scavenges ROS and may improve the ability of OE9 plants to remove ROS under drought conditions . This hypothesis was supported by the patterns of ROS accumulation in the various lines (Fig. 5d). Thus, the increased drought tolerance of the OE9 plants was related to increased transcript levels of genes that are normally drought-repressed genes in WT plants under drought stress.
Surprisingly, the 207 DIG-Ds included many stress-responsive transcription factors, including MYB, WRKY and basis helix–loop–helix (bHLH) types. Specifically, these included MYB90, which regulates anthocyanin synthase, and affects anthocyanin accumulation under adverse conditions ; and bHLH38 and bHLH39, which are involved in improving iron homeostasis . The DIG-Ds also included drought-related genes, such as LTP3, LTP4, and COR413IM1; and the ABA signaling-regulated gene AFP3. Because the transcript levels of genes that are drought-induced in WT were downregulated in the OE9 plants, we hypothesized that the overexpression of AtTPPF contributed to drought responses by stimulating vigorous growth during drought. To verify the RNA-seq data, we performed quantitative RT-PCR to measure the expression levels of several of the annotated genes (Fig. 6c). The results were consistent with those obtained from the RNA-seq data.
AtTPPF increases drought tolerance through accumulation of soluble sugars
Previous studies on the TPP family have focused mainly on their functions in Arabidopsis growth and development and in improving crop production [2, 16, 21]. They have been the subject of practical field trials in certain crop species, including wheat and rice [19, 20]. For example, the OsTPP1 gene was shown to increase drought tolerance and yields when overexpressed in maize . As a member of the TPP family in Arabidopsis, the AtTPPF gene is highly homologous to OsTPP1, which is induced specifically under dehydration conditions . In this study, the expression of AtTPPF was induced by drought (Fig. 3a). The loss of function of AtTPPF led to drought sensitivity, while its overexpression increased drought tolerance (Figs. 1 and 2). These results indicate that AtTPPF plays a positive role in drought tolerance.
Trehalose is a non-reducing disaccharide that functions as an osmoprotectant in the maintenance of cellular osmotic balance . Trehalose is thought to act as an osmolyte that protects resurrection plants, such as Selaginella spp.  and Myrothamnus flabellifolius , from desiccation. Under dehydration conditions, trehalose plays a role in stabilizing dehydration-related enzymes and proteins, as well as lipid membranes, and it can scavenge ROS to protect biological structures from damage . As a non-reducing disaccharide, trehalose is stable and unreactive. It accumulates to high levels in fungi, bacteria, insects, and arthropods, in which it plays a dual role as a carbon storage molecule and a protective compound in certain situations. Trehalose may also be a target to improve drought tolerance in plants. Increasing the expression of TPP in Arabidopsis led to a four-fold increase in trehalose contents [1, 36]. Our results showed that the trehalose content is very low in Arabidopsis (less than 10 μg/g FW, even under drought conditions), consistent with a previous report . The loss of function of AtTPPF did not affect the trehalose content under either well-watered or drought conditions, consistent with a previous study . This may result from the complementary effects of other genes, such as AtTPPG (the duplicate of AtTPPF). However, the overexpression of AtTPPF resulted in a 25% increase in the trehalose content under drought conditions, compared with the level in WT plants (Fig. 5a). Even though we detected a slight effect of AtTPPF as an osmotic protector, the OE9 plants showed increased ROS scavenging abilities (Fig. 5d). The enhanced soluble sugars level under drought conditions may also contribute to osmotic protection (Fig. 5e). Thus, the overexpression of AtTPPF increased the levels of soluble sugars, which could subsequently protect the cell from oxidative damage and enhance drought tolerance.
AtTPPF-overexpressing plants maintain carbohydrate metabolism and exhibit decreased drought sensitivity under drought stress
We detected no correlation between the prominent drought-tolerant phenotype and limited trehalose accumulation, suggesting that some other pathway might be responsible for the increased drought tolerance conferred by AtTPPF. Therefore, we performed a comparative transcriptional analysis of OE9 and WT plants under well-watered and drought conditions. The overexpression of AtTPPF altered the transcript levels of numerous genes encoding transcription factors and enzymes under drought conditions (Fig. 6b). Among the ERG-Us, 318 were upregulated in the OE9 plants during drought stress. They encoded proteins involved in electron transport activity, carbohydrate metabolism, cell wall modification, and ROS scavenging. Thus, the overexpression of AtTPPF may contribute to increasing photosynthesis, protecting biological components from damage caused by ROS, and maintaining carbohydrate metabolism under drought conditions. The 207 genes that were downregulated in the OE9 plants under drought stress included numerous stress-related transcription factor genes and drought-related genes. This result indicates that the increased expression of AtTPPF reduces the negative effects of drought stress by some unknown mechanism. Considering the relatively low accumulation of H2O2 and the relatively low expression levels of drought-induced regulators in OE9 plants under drought, this relief of the negative effects of drought may partly result from ROS removal, which would improve the osmotic regulation of OE9 plant cells. Thus, increasing the expression of AtTPPF could maintain the balance between gene expression and metabolism, as well as increasing adaptability to drought conditions.
AtTPPF is a direct downstream target of DREB1A
Although the overexpression of TPS and/or TPP has been widely used to increase plant drought tolerance [6, 19, 20, 36], the underlying molecular mechanisms involved in the dynamic regulation of genes in the trehalose biosynthetic pathway under drought-stress conditions are largely unknown. DREB1A is a DREB/CBF transcription factor that interacts specifically with the DRE/CRT cis-acting element and regulates the expression of many stress-inducible genes to increase the stress tolerance of Arabidopsis . In Arabidopsis, DREB1A expression can improve abiotic stress tolerance by increasing late embryogenesis-abundant protein levels and compatible solute contents [22, 38]. In another study, trehalose levels were significantly increased in WT and 35S:DREB1A transgenic plants grown under drought conditions for 2–3 d . Here, the expression of AtTPPF during drought stress was consistent with that of DREB1A (Fig. 3a, b). Yeast one-hybrid assays revealed that DREB1A could bind directly to the drought-responsive DRE/CRT element in the AtTPPF promoter, and LUC activity assays further indicated that DREB1A could positively activate AtTPPF gene expression. Furthermore, the transcript abundance of AtTPPF was significantly lower in the dreb1a mutant and much higher in the DREB1A-OX plants than in WT (Fig. 4e, f). Although the expression of AtTPPF was also induced in the dreb1a mutant under drought conditions, this phenomenon may result from the existence of other modulatory genes. Thus, AtTPPF appears to be a direct downstream target of DREB1A, and AtTPPF expression is positively modulated by DREB1A in plants.
We developed a hypothetical response model for AtTPPF during drought stress (Fig. 7). Under normal conditions, there is sufficient water to meet all the needs of the plant, and the stress-induced gene DREB1A is in the “OFF” position. When the plant encounters drought stress, DREB1A switches to the “ON” position. DREB1A is expressed at high levels, and binds to the DRE/CRT motif in the promoter of AtTPPF and activates its transcription. The increased expression of AtTPPF most likely affects the T6P signal, which may lead to an increase in soluble sugars. Soluble sugars function as osmolytes to protect the cells of young leaves from ROS damage and allow them to complete their life cycle normally (Fig. 7).
Our results show that AtTPPF is related to changes in soluble sugar levels under drought conditions. AtTPPF-overexpressing plants show increased levels of soluble sugars, which may function as osmolytes to protect cells from ROS damage. Our results also show that DREB1A can directly interact with the AtTPPF promoter and activate AtTPPF transcription under drought conditions. These findings shed light on the transcriptional regulation of AtTPPF and also provide a practical tool for improving the drought tolerance of plants through the manipulation of AtTPPF expression.
Plant materials and growth conditions
The ecotype used in the article is Col-0. T-DNA insertion loss-of-function tppf1 mutant seeds (SALK_087220) were kindly supplied by Professor Yuan Qin from Center for Genomics and Biotechnology, Haixia Institute of Science and Technology of Fujian Agriculture and Forestry University. Seeds of the dreb1a T-DNA insertion line (SALK_018603) and the DREB1A-OX line (CS69502) were ordered from the Arabidopsis Biological Resource Center (ABRC). Single-copy and homozygous T3 plants were identified by genetic segregation on agar medium that contained kanamycin. Stratified seeds were transferred either to Murashige and Skoog (MS) medium or to the soil and cultivated at 22 °C under a 16-h light/8-h dark photoperiod at 80 μmol quanta m− 2 s− 1 light intensity and 50% relative humidity.
For tppf1 T-DNA insertion identification, total genomic DNA was extracted from the leaves, and PCR was performed using primer pairs: LP: 5′-TTCACCTGCACACAACAAATC-3′, RP: 5′-CCCAACATGGATGGTTCTTC-3′ and LB: 5′-ATTTTGCCGATTTCGGAAC-3′.
For the identification of dreb1a T-DNA insertion identification, the PCR primer pairs used are: LP: 5′-GCCACACATTCATACGCAAAGA-3′, RP:5′- AGCTCGAGCTGCCATCTCAGCG-3′and LB: 5′-GCGTGGACCGCTTGCTGCAACT-3′.
Drought stress analysis
One-week-old wild-type (Col-0), 35S:AtTPPF transgenic, tppf1 mutant plants and the complementary plants grown in soil (PINDSTRUP from Denmark, pH 5.0, 0–10 mm, using the same weight of black plastic pot 10 cm × 10 cm, dry weight of soil: 100 g ± 0.1 g, and make sure the filled water is the same) in a growth room (16 h light/8 h dark, 22 °C, 50% humidity) were subjected to drought stress by withholding water for approximately 26 d (the duration ranged from 14 to 26 d according to the growth conditions and the number of seedlings per container in each experiment, for the tppf1 mutant drought assay, 4 seedlings planted in a container, the drought days are 28 d; for the 35S:AtTPPF transgenic lines drought assay, 9 seedlings planted in a container, the drought days are 26 d; and for the complementary experiments, 5 seedlings planted in a container, the drought days are 14 d). Once differences were apparent in each experiment, the plants were re-watered, after which their survival rates were determined after 2 d.
Plasmid constructs and plant transformation
Total RNA was extracted from 2-week-old Col-0 seedlings with TRIzol (RNAiso plus, 9109). First-strand cDNA was synthesized with an 18-mer oligo (dT) primer. Subsequent PCR amplification of AtTPPF cDNA involved the primer pair TPPF-F and TPPF-R (Additional file 5: Table S1).
The PCR products were fused to the Cauliflower mosaic virus (CaMV) 35S promoter, which was then inserted into a pMDC140 binary vector with Gateway Technology (Invitrogen). The constructs were introduced into Agrobacterium tumefaciens strain GV3101 and transformed via floral infiltration into Col-0 (for overexpression analysis) and tppf1 mutants (for complementation experiment).
RT-PCR and RT-qPCR analysis
Total RNA was isolated from two-week-old seedlings using TRIzol (Takara, RNAiso plus, 9109). First-strand cDNA was synthesized. The volume of each cDNA pool was adjusted to give the same PCR signal strength for eIf-4A after 28 cycles. The RT-PCR products were analyzed by electrophoresis on a 1.0% agarose gel. All PCRs were performed in triplicate.
RT-qPCR analysis was performed with a SYBR Premix Ex Taq Kit (TaKaRa) using a CFX96 Real-time PCR Detection System (Bio-Rad), and eIF-4A was used as the internal standard. The RT-qPCR primers are given in Additional file 6: Table S2.
Estimation of trehalose contents
Trehalose content is estimation in accordance with the manufacturer’s protocol (Solarbio, BC0330). The principle of this method is the same as that of the determination of soluble sugar –“Anthrone colorimetry”. The only difference is the application of standard substance. The trehalose (10 mg/mL) was used for the drawing of standard curve in trehalose content measurements. However, this method has some limitations and only could estimate trehalose content because the signal produced by other soluble sugars in plants might affect the determination. Two-week-old seedlings grown on MS medium were treated with or without 20% polyethylene glycol (PEG) for 4 h, approximately 0.1 g leaf tissue was sampled to determine the trehalose content. The sample was ground in liquid nitrogen and 1 mL extraction buffer was added for homogenization. After incubating for 45 min at room temperature (the homogenate was mixed 3–5 times during the incubation period), the sample was centrifuged at 8000×g at 25 °C for 10 min. The supernatant was used for the determination of trehalose content. First, draw the standard curve with the standard trehalose solution (10 mg/mL). Mix 0.25 mL trehalose standard liquid of different concentration with 1 mL anthrone dissolved in 80% H2SO4 and then keep in water bath at 95 °C for 10 min, and the absorbance of the mixture was recorded at 620 nm. The primed sample supernatant was determined by the same method. Trehalose content is calculated from a standard curve of trehalose at 620 nm.
Analysis of H2O2 accumulation
H2O2 accumulation was measured using DAB staining. For DAB staining assays, seedlings grown on MS plates for 10 d were left untreated or treated with PEG6000 for 24 h and then incubated in staining buffer (0.1 mg/mL DAB dissolved in 0.1 M HCl, pH 3.8). After 12 h, seedlings were distained in 75% ethanol for subsequent microscopy.
Soluble sugar content measurement
The total soluble sugar measurements was performed as described as the reference . Ten-day-old seedlings on a plastic slide were treated with or without 20% PEG for 1 d, after which approximately 0.1 g of leaf tissue was sampled for the determine of the soluble sugar. The sample of 0.1 g was collected and homogenization in 2.5 mL ddH2O. Boiled for 15 min, and after cooled at room temperature, centrifuged at 12,000 rpm for 15 min. Then the supernatant was used for the determination. Mix 50 μL supernatant with the 2.5 mL anthrone regent and boiled them for 10 min, cooled at room temperature and then the absorbance of the mixture was recorded at 620 nm. The content of soluble sugar content is calculated from a standard curve of glucose at 620 nm.
Sucrose content measurement
Sucrose content was determined by the plant tissue sucrose content detection kit (Solarbio, BC2465). Ten-day-old seedlings on a plastic slide were treated with or without 20% PEG for 1 d, after which approximately 0.1 g of leaf tissue was sampled for the sucrose measurements. After the sample was grounded to powder, 0.5 mL extraction buffer was added and incubated it at 80 °C for 10 min. After which, the sample was destained and centrifuged at 4000×g at 25 °C for 10 min. The fundamental method was resorcinol method and the protocol was similar with the reference . 50 μL of the aforementioned supernatant was added into 50 μL 2 M NaOH, boiled for 5 min at 100 °C, cooled down at room temperature, then mixed with 350 μL HCl and 100 μL 0.1% resorcinol. Then the mixture was water bathed at 100 °C for 10 min and was cooled down to room temperature. The content of sucrose was measured at A480.
Yeast one-hybrid assay
The yeast one-hybrid assay was done according to the Matchmaker One-Hybrid System User Manual (Clontech). Plasmid pJG4–5 was used to express DREB1A to create the prey vector. Reporter plasmid pLacZi-2 μ was modified from pLacZi (Clontech) as previously described; it is a high copy autonomous vector . To construct the prey vector, the DREB1A coding sequence was amplified by PCR from Phanta Super-Fidelity DNA Polymerase (Vazyme) and cloned into the pJG4–5 vector through ClonExpress II One Step Cloning Kit (Vazyme, C112–01) using the linearization of pJG4–5 with EcoRI and XhoI site. Primers with adaptor were DREB1A-F and DREB1A-R (Additional file 5: Table S1). To create the bait vectors P1-pLaCZi-2 μ, the P1 promoter regions containing the DRE/ARE cis-element. P1 were amplified via PCR with P1-F and P1-R (Additional file 5: Table S1) using genome DNA template of wild-type. The PCR product was introduced into the pLacZi-2 μ vector with the ClonExpress II One Step Cloning Kit using the linearization of pLacZi-2 μ with EcoRI and KpnI site. The two construct was co-transformed into the yeast strain EGY48 through the protocol described in the Matchmaker One-Hybrid System User Manual and selected on the respective selective dropout media (SD) lacking uracil and tryptophan and supplemented with X-Gal. Each interaction was tested three times in parallel in three independent experiments.
Dual luciferase reporter assays
The full-length DREB1A open reading frame (ORF) was amplified from WT cDNA via the gene-specific primers DREB1A-F1 and DREB1A-R1 (Additional file 5: Table S1) and cloned into the effector vector pGreenII62-SK  under the control of the CaMV 35S. A 539-bp AtTPPF P1 promoter fragment was amplified with the specific primers P1-F1 and P1-R1 (Additional file 5: Table S1) and then ligated into the reporter vector, pGreen II 0800-LUC .
The effector and reporter constructs were transformed into GV3101 via the helper plasmid pSoup-P19. Agrobacterium tumefaciens GV3101 harboring the above plasmids were then infiltrated into tobacco plants in the following combinations and ratios: LUC + 35S:DREB1A(1:9); P1:LUC + 62-SK (1:9); P1:LUC+ 35S:DREB1A (1:9); The infiltrated tobacco plants were grown for an additional 3 d in a growth chamber under a 16-h light/8-h dark photoperiod at 21 °C. Afterward, 2-cm-diameter leaf discs were harvested and ground for luciferase and Renilla assays via the Dual-Luciferase Reporter Assay System (Promega) with an Infinite200 Pro microplate reader (Tecan). The promoter activity was expressed as the ratio of LUC to REN.
RNA sequencing (RNA-seq) and analysis
Total RNA was extracted from two-week-old seedlings using RNeasy Plant Mini Kit (QIAGEN 74904). Two micrograms of RNA were used for library construction; each sample was replicated three times. The transcriptome data set used in this study was obtained using the Illumina HiSeq platform, and 150-bp high-quality trimmed paired-end reads were generated. The trimmed reads were mapped to the reference genome sequence of Arabidopsis using HISAT2 (http://0-ccb.jhu.edu.brum.beds.ac.uk/software/hisat2/faq.shtml), with the default settings . Differentially expressed genes were analyzed using edgeR (http://bioinf.wehi.edu.au/edgeR/) . Genes were considered differentially expressed if their change in expression was more than twofold that of WT (P < 0.05).
Availability of data and materials
The datasets generated and analyzed during the current study are available from the corresponding author on reasonable request.
Cauliflower mosaic virus
Mitogen-activated protein kinase
Open reading frame
Reactive oxygen species
Reverse transcription polymerase chain reaction
Shoot apical meristem
Avonce N, Mendoza-Vargas A, Morett E, Iturriaga G. Insights on the evolution of trehalose biosynthesis. BMC Evol Biol. 2006;6:109.
Paul MJ, Gonzalez-Uriarte A, Griffiths CA, Hassani-Pak K. The role of Trehalose 6-phosphate in crop yield and resilience. Plant Physiol. 2018;177(1):12–23.
Figueroa CM, Lunn JE. A tale of two sugars: Trehalose 6-phosphate and sucrose. Plant Physiol. 2016;172(1):7–27.
O'Hara LE, Paul MJ, Wingler A. How do sugars regulate plant growth and development? New insight into the role of trehalose-6-phosphate. Mol Plant. 2013;6(2):261–74.
Wingler A, Delatte TL, O'Hara LE, Primavesi LF, Jhurreea D, Paul MJ, Schluepmann H. Trehalose 6-phosphate is required for the onset of leaf senescence associated with high carbon availability. Plant Physiol. 2012;158(3):1241–51.
Gomez LD, Gilday A, Feil R, Lunn JE, Graham IA. AtTPS1-mediated trehalose 6-phosphate synthesis is essential for embryogenic and vegetative growth and responsiveness to ABA in germinating seeds and stomatal guard cells. Plant J. 2010;64(1):1–13.
Chary SN, Hicks GR, Choi YG, Carter D, Raikhel NV. Trehalose-6-phosphate synthase/phosphatase regulates cell shape and plant architecture in Arabidopsis. Plant Physiol. 2008;146(1):97–107.
Schluepmann H, van Dijken A, Aghdasi M, Wobbes B, Paul M, Smeekens S. Trehalose mediated growth inhibition of Arabidopsis seedlings is due to trehalose-6-phosphate accumulation. Plant Physiol. 2004;135(2):879–90.
Chen ZH, Wang Y, Wang JW, Babla M, Zhao C, Garcia-Mata C, Sani E, Differ C, Mak M, Hills A, et al. Nitrate reductase mutation alters potassium nutrition as well as nitric oxide-mediated control of guard cell ion channels in Arabidopsis. New Phytol. 2016;209(4):1456–69.
Luo Y, Li WM, Wang W. Trehalose: protector of antioxidant enzymes or reactive oxygen species scavenger under heat stress? Environ Exp Bot. 2008;63(1):378–84.
Couee I, Sulmon C, Gouesbet G, El Amrani A. Involvement of soluble sugars in reactive oxygen species balance and responses to oxidative stress in plants. J Exp Bot. 2006;57(3):449–59.
Krasensky J, Broyart C, Rabanal FA, Jonak C. The redox-sensitive chloroplast trehalose-6-phosphate phosphatase AtTPPD regulates salt stress tolerance. Antioxid Redox Signal. 2014;21(9):1289–304.
Nunes C, Primavesi LF, Patel MK, Martinez-Barajas E, Powers SJ, Sagar R, Fevereiro PS, Davis BG, Paul MJ. Inhibition of SnRK1 by metabolites: tissue-dependent effects and cooperative inhibition by glucose 1-phosphate in combination with trehalose 6-phosphate. Plant Physiol Biochem. 2013;63:89–98.
Lunn JE, Feil R, Hendriks JH, Gibon Y, Morcuende R, Osuna D, Scheible WR, Carillo P, Hajirezaei MR, Stitt M. Sugar-induced increases in trehalose 6-phosphate are correlated with redox activation of ADPglucose pyrophosphorylase and higher rates of starch synthesis in Arabidopsis thaliana. Biochem J. 2006;397(1):139–48.
Delorge I, Figueroa CM, Feil R, Lunn JE, Van Dijck P. Trehalose-6-phosphate synthase 1 is not the only active TPS in Arabidopsis thaliana. Biochem J. 2015;466(2):283–90.
Vandesteene L, Lopez-Galvis L, Vanneste K, Feil R, Maere S, Lammens W, Rolland F, Lunn JE, Avonce N, Beeckman T, et al. Expansive evolution of the trehalose-6-phosphate phosphatase gene family in Arabidopsis. Plant Physiol. 2012;160(2):884–96.
Vandesteene L, Ramon M, Le Roy K, Van Dijck P, Rolland F. A single active trehalose-6-P synthase (TPS) and a family of putative regulatory TPS-like proteins in Arabidopsis. Mol Plant. 2010;3(2):406–19.
Avonce N, Leyman B, Mascorro-Gallardo JO, Van Dijck P, Thevelein JM, Iturriaga G. The Arabidopsis trehalose-6-P synthase AtTPS1 gene is a regulator of glucose, abscisic acid, and stress signaling. Plant Physiol. 2004;136(3):3649–59.
Zhang P, He Z, Tian X, Gao F, Xu D, Liu J, Wen W, Fu L, Li G, Sui X, et al. Cloning of TaTPP-6AL1 associated with grain weight in bread wheat and development of functional marker. Mol Breed. 2017;37(6).
Nuccio ML, Wu J, Mowers R, Zhou H-P, Meghji M, Primavesi LF, Paul MJ, Chen X, Gao Y, Haque E, et al. Expression of trehalose-6-phosphate phosphatase in maize ears improves yield in well-watered and drought conditions. Nat Biotechnol. 2015;33(8):862–9.
Van Houtte H, Lopez-Galvis L, Vandesteene L, Beeckman T, Van Dijck P. Redundant and non-redundant roles of the trehalose-6-phosphate phosphatases in leaf growth, root hair specification and energy-responses in Arabidopsis. Plant Signal Behav. 2013;8(3):e23209.
Maruyama K, Takeda M, Kidokoro S, Yamada K, Sakuma Y, Urano K, Fujita M, Yoshiwara K, Matsukura S, Morishita Y, et al. Metabolic pathways involved in cold acclimation identified by integrated analysis of metabolites and transcripts regulated by DREB1A and DREB2A. Plant Physiol. 2009;150(4):1972–80.
Riechmann JL, Meyerowitz EM. The AP2/EREBP family of plant transcription factors. Biol Chem. 1998;379(6):633–46.
Van Houtte H, Vandesteene L, Lopez-Galvis L, Lemmens L, Kissel E, Carpentier S, Feil R, Avonce N, Beeckman T, Lunn JE, et al. Overexpression of the trehalase gene AtTRE1 leads to increased drought stress tolerance in Arabidopsis and is involved in abscisic acid-induced stomatal closure. Plant Physiol. 2013;161(3):1158–71.
Luna CM, Pastori GM, Driscoll S, Groten K, Bernard S, Foyer CH. Drought controls on H2O2 accumulation, catalase (CAT) activity and CAT gene expression in wheat. J Exp Bot. 2005;56(411):417–23.
Kim D, Langmead B, Salzberg SL. HISAT: a fast spliced aligner with low memory requirements. Nat Methods. 2015;12(4):357–60.
Kim TH, Bohmer M, Hu H, Nishimura N, Schroeder JI. Guard cell signal transduction network: advances in understanding abscisic acid, CO2, and Ca2+ signaling. Annu Rev Plant Biol. 2010;61:561–91.
Endo A, Tatematsu K, Hanada K, Duermeyer L, Okamoto M, Yonekura-Sakakibara K, Saito K, Toyoda T, Kawakami N, Kamiya Y, et al. Tissue-specific transcriptome analysis reveals cell wall metabolism, flavonol biosynthesis and defense responses are activated in the endosperm of germinating Arabidopsis thaliana seeds. Plant Cell Physiol. 2012;53(1):16–27.
Myouga F, Hosoda C, Umezawa T, Iizumi H, Kuromori T, Motohashi R, Shono Y, Nagata N, Ikeuchi M, Shinozaki K. A heterocomplex of iron superoxide dismutases defends chloroplast nucleoids against oxidative stress and is essential for chloroplast development in Arabidopsis. Plant Cell. 2008;20(11):3148–62.
Bac-Molenaar JA, Fradin EF, Rienstra JA, Vreugdenhil D, Keurentjes JJ. GWA mapping of anthocyanin accumulation reveals balancing selection of MYB90 in Arabidopsis thaliana. PLoS One. 2015;10(11):e0143212.
Kurt F, Filiz E. Genome-wide and comparative analysis of bHLH38, bHLH39, bHLH100 and bHLH101 genes in Arabidopsis, tomato, rice, soybean and maize: insights into iron (Fe) homeostasis. Biometals. 2018;31(4):489–504.
Elbein AD, Pan YT, Pastuszak I, Carroll D. New insights on trehalose: a multifunctional molecule. Glycobiology. 2003;13(4):17R–27R.
Zentella R, Mascorro-Gallardo JO, Van Dijck P, Folch-Mallol J, Bonini B, Van Vaeck C, Gaxiola R, Covarrubias AA, Nieto-Sotelo J, Thevelein JM, et al. A Selaginella lepidophylla trehalose-6-phosphate synthase complements growth and stress-tolerance defects in a yeast tps1 mutant. Plant Physiol. 1999;119(4):1473–82.
Goddijn O, Smeekens S. Sensing trehalose biosynthesis in plants. Plant J. 1998;14(2):143–6.
Garg AK, Kim JK, Owens TG, Ranwala AP, Choi YD, Kochian LV, Wu RJ. Trehalose accumulation in rice plants confers high tolerance levels to different abiotic stresses. Proc Natl Acad Sci U S A. 2002;99(25):15898–903.
Miranda JA, Avonce N, Suarez R, Thevelein JM, Van Dijck P, Iturriaga G. A bifunctional TPS-TPP enzyme from yeast confers tolerance to multiple and extreme abiotic-stress conditions in transgenic Arabidopsis. Planta. 2007;226(6):1411–21.
Liu Q, Kasuga M, Sakuma Y, Abe H, Miura S, Yamaguchi-Shinozaki K, Shinozaki K. Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell. 1998;10(8):1391–406.
Maruyama K, Sakuma Y, Kasuga M, Ito Y, Seki M, Goda H, Shimada Y, Yoshida S, Shinozaki K, Yamaguchi-Shinozaki K. Identification of cold-inducible downstream genes of the Arabidopsis DREB1A/CBF3 transcriptional factor using two microarray systems. Plant J. 2004;38(6):982–93.
Zhang H, Liu H, Yang R, Xu X, Liu X, Xu J. Over-expression of PttEXPA8 gene showed various resistances to diverse stresses. Int J Biol Macromol. 2019;130:50–7.
Liu H, Wang Q, Yu M, Zhang Y, Wu Y, Zhang H. Transgenic salt-tolerant sugar beet (Beta vulgaris L.) constitutively expressing an Arabidopsis thaliana vacuolar Na/H antiporter gene, AtNHX3, accumulates more soluble sugar but less salt in storage roots. Plant Cell Environ. 2008;31(9):1325–34.
Feschotte C, Osterlund MT, Peeler R, Wessler SR. DNA-binding specificity of rice mariner-like transposases and interactions with stowaway MITEs. Nucleic Acids Res. 2005;33(7):2153–65.
Hellens RP, Allan AC, Friel EN, Bolitho K, Grafton K, Templeton MD, Karunairetnam S, Gleave AP, Laing WA. Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants. Plant Methods. 2005;1:13.
Robinson MD, McCarthy DJ, Smyth GK. edgeR: a Bioconductor package for differential expression analysis of digital gene expression data. Bioinformatics. 2010;26(1):139–40.
We thank Dr. Yuan Qin and Dr. Liyu Chen for supplying with AtTPPF T-DNA insertion line (SALK_087220) and Wassilewskija (WS) seeds.
This work was supported by the National Natural Science Foundation of China (31771862 and 31471170) and National Engineering Research Center of Sugarcane Open Fund (2017.1.5). The funding bodies were not involved in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript.
Ethics approval and consent to participate
Consent for publication
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Figure S1. Phylogenetic analysis of the TPP genes from Arabidopsis and Rice. The deduced full-length amino acid sequences of 10 members in Arabidopsis, and 10 members in Rice TPP proteins respectively were aligned by MUSCLE 3.8 and the phylogenetic tree was constructed using MEGA 7.0 by the Neighbor-Joining (NJ) method with 1000 bootstrap replicates. Each TPP subfamily has been separated and is depicted using different colors. The black circle is the Arabidopsis AtTPPF. (TIF 986 kb)
Figure S2. Complementation assays of the tppf1 mutant. Analysis of the drought-tolerant phenotype of plants of a complementary line Com1. Two-week-old plants growing in the soil were subjected to dehydration by withholding water for 19 d, after which the image shown was taken. Three independent assays were performed, each of which produced similar results. (TIF 7106 kb)
Figure S3. Coexpression model of AtTPPF and AtDREB1A under abiotic stress conditions. Coexpression model of AtTPPF and AtDREB1A under abiotic stress conditions developed using the AtGenExpress Visualization Tool (AVT) on TAIR (http://jsp.weigelworld.org/expviz/expviz.jsp?experiment). At4g12430 is AtTPPF, and At4g25480 is AtDREB1A. The intensity indicates the corresponding expression value. (TIF 5542 kb)
Figure S4. Autonomous activated verification of AtTPPF-P and the interaction of AREB1 and AtTPPF-P1. Results of Y1H assays and the growth of yeast cells, AtTPPF-P: full-length promoter (P) of the AtTPPF gene cotransformed with the pJG4–5 empty vector; AtTPPF-P + AREB1: bait full-length promoter (P) of the AtTPPF gene cotransformed with prey AREB1; AtTPPF-P1: bait P1 cotransformed with the pJG4–5 empty vector; AtTPPF-P1 + AREB1: bait P1 cotransformed with prey AREB1 on selective medium supplemented with X-Gal. (TIF 2662 kb)
Table S1. Primers for vector construction. (DOC 33 kb)
Table S2. Primers for the qRT-PCR. (DOC 44 kb)
Table S3. The information of 318 drought-repressed genes which up-regulated in OE plants (ERG-U). (XLS 59 kb)
Table S4. The information of 207 drought-induced genes which down-regulated in OE plants (DIG-D). (XLS 40 kb)
About this article
Cite this article
Lin, Q., Yang, J., Wang, Q. et al. Overexpression of the trehalose-6-phosphate phosphatase family gene AtTPPF improves the drought tolerance of Arabidopsis thaliana. BMC Plant Biol 19, 381 (2019). https://0-doi-org.brum.beds.ac.uk/10.1186/s12870-019-1986-5
- Arabidopsis thaliana
- Drought tolerance