Table 3 Primer sequences and efficiency of the shortlisted primers used in this study
Gene.Name | Primers (5′ to 3′) | Efficiency (%) | R 2 |
|---|---|---|---|
PP2A1 | F-GATCCTTGTGGAGGAGTGGA | 93.54 | 0.99 |
R-GCGAAACAGTTCGACGAGAT | |||
TMN5 | F-CTCACCATTCCATTACTTGTGTTG | 103.24 | 0.97 |
R-GAGGAATCTCTCTCGGGTATCT | |||
UBQ14 | F-CAACGCTCCATCTTGTCCTT | 103.74 | 0.99 |
R-TGATCGTCTTTCCCGTAAGC | |||
TBL6 | F-AGCAGATCCAGAGACAAGAAAG | 95.14 | 0.99 |
R-CCATTGTAGGTGCAGGTGTAT | |||
UTR5B | F-CGGTCTCTGCTGGTTCTTTAG | 94.91 | 0.99 |
R-TGACATGTTGTGGTTAGGATGT | |||
At1g65240 | F-GCAAACTCTACAGCTCCCATTA | 104.42 | 0.99 |
R-GTCCAAACCCGAAGATTCCA | |||
CYP76B6 | F-TGGCTTGGATGCCTGTTT | 103.71 | 0.99 |
R-TCGCCGTAAGTGTTGGTTAG |