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Fig. 3 | BMC Plant Biology

Fig. 3

From: A novel R2R3-MYB from grape hyacinth, MaMybA, which is different from MaAN2, confers intense and magenta anthocyanin pigmentation in tobacco

Fig. 3

Bimolecular fluorescence complementation (BiFC) assay and dual luciferase assay. a BiFC assay was performed to confirm the interaction between MabHLH1 and MaMybA or MaAN2 in Nicotiana benthamiana leaves. Bars: 25 μm. b Dual luciferase assay of MabHLH1 with or without MaMybA or MaAN2: effects on the transcriptional regulation of MaCHS, MaDFR, and AtDFR promoters, respectively, in N. benthamiana leaves. The results show promoter activity expressed as the ratio of the MaCHS, MaDFR, and AtDFR promoters, LUC to 35S: REN. Error bars indicate the standard deviations (SD) of average results

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