Skip to main content

Table 4 Changes of LN stress on metabolites content in old leaves of two wild soybean varieties

From: Physiological and metabolomics analyses of young and old leaves from wild and cultivated soybean seedlings under low-nitrogen conditions

Metabolite name

Relative concentration

Fold changes Log2(LN/CK)

C

W

CK

LN

CK

LN

C

W

Aliphatic

amino acid

Asparagine

0.51 ± 0.06

0.06 ± 0.02

0.06 ± 0.01

0.22 ± 0.05

−3.20**

1.95*

Aspartic acid

1.53 ± 0.33

0.79 ± 0.24

0.07 ± 0.04

0.33 ± 0.09

−0.95

2.28*

Threonine

1.43 ± 0.03

1.24 ± 0.15

0.02 ± 0.00

0.03 ± 0.01

−0.20

0.97*

Cycloleucine

0.85 ± 0.06

1.31 ± 0.55

30.89 ± 5.31

44.38 ± 8.78

0.63

0.52

Isoleucine

5.58 ± 0.20

2.58 ± 0.19

0.38 ± 0.01

1.33 ± 0.25

−1.11

1.79**

L − proline

2.46 ± 1.02

5.06 ± 0.68

0.11 ± 0.01

0.81 ± 0.18

1.04

2.85**

L − homoserine

0.29 ± 0.05

0.33 ± 0.02

1.04 ± 0.21

2.42 ± 0.26

0.19

1.22**

Valine

5.63 ± 0.87

4.42 ± 0.09

37.13 ± 3.86

47.46 ± 5.60

−0.35*

0.35

Alanine

2.46 ± 0.32

1.61 ± 0.52

0.09 ± 0.01

0.26 ± 0.03

−0.61

1.57**

Glycine

3.51 ± 0.54

2.62 ± 0.46

1.07 ± 0.15

1.27 ± 0.30

−0.42*

0.25

Serine

8.50 ± 0.84

12.51 ± 2.47

0.01 ± 0.00

0.06 ± 0.01

0.56

2.32*

Sarcosine

1.59 ± 0.17

2.07 ± 0.23

0.81 ± 0.15

2.97 ± 0.63

0.38

1.88*

Aromatic amino acid

Phenylalanine

0.71 ± 0.21

0.49 ± 0.04

0.08 ± 0.03

0.17 ± 0.02

−0.53

1.01*

Non − protein nitrogen

Galactosamine

0.08 ± 0.01

0.10 ± 0.00

9.79 ± 4.25

17.95 ± 3.79

0.26

0.87

GABA

8.34 ± 0.78

6.38 ± 1.42

0.10 ± 0.02

0.19 ± 0.02

−0.39

0.92*

Maleimide

0.93 ± 0.08

0.77 ± 0.18

0.23 ± 0.01

0.31 ± 1.10

−0.27

0.43*

Mannosamine

0.83 ± 0.09

1.06 ± 0.08

3.67 ± 0.66

10.39 ± 0.63

0.36

1.50**

Ethanolamine

19.62 ± 0.57

20.71 ± 4.45

30.85 ± 6.18

61.33 ± 6.31

0.08

0.99*

Sugars and polyols

Glycerol

14.12 ± 0.18

11.49 ± 0.27

0.10 ± 0.01

0.20 ± 0.00

−0.30

0.95**

Monopalmitin

0.04 ± 0.01

0.16 ± 0.05

25.16 ± 8.47

106.06 ± 10.98

1.83

2.08

Myo − inositol

19.77 ± 0.48

28.56 ± 1.03

0.60 ± 0.07

1.48 ± 0.41

0.53**

1.31**

Palatinitol

0.04 ± 0.00

0.06 ± 0.00

0.29 ± 0.06

0.52 ± 0.11

0.78**

0.85

Arabitol

1.46 ± 0.14

1.57 ± 0.06

0.12 ± 0.01

0.57 ± 0.10

0.10

2.23**

Threitol

0.84 ± 0.23

1.25 ± 0.14

0.17 ± 0.01

0.37 ± 0.06

0.57

1.15*

Erythritol

0.08 ± 0.02

0.20 ± 0.03

1.23 ± 0.25

4.26 ± 0.15

1.24**

1.79*

Sucrose

36.97 ± 1.51

29.08 ± 1.92

30.81 ± 9.34

2.64 ± 0.02

−0.35**

−3.54*

Maltose

3.03 ± 0.42

2.37 ± 0.32

0.12 ± 0.01

0.22 ± 0.05

−0.36

0.91

Melezitose

0.24 ± 0.11

0.23 ± 0.02

0.004 ± 0.00

0.03 ± 0.00

−0.11

2.77**

L − threose

0.12 ± 0.03

0.12 ± 0.01

4.15 ± 0.23

4.96 ± 0.75

−0.04

0.26

Fucose

0.17 ± 0.06

0.10 ± 0.00

0.08 ± 0.00

0.12 ± 0.01

−0.81

0.68**

Xylose

0.22 ± 0.03

0.37 ± 0.03

6.36 ± 0.17

10.71 ± 2.81

0.75*

0.75

Galactose

0.87 ± 0.20

1.70 ± 0.15

0.05 ± 0.01

0.08 ± 0.01

0.96*

0.53

Fructose-6 − biphosphate

0.06 ± 0.01

0.22 ± 0.04

0.06 ± 0.00

0.15 ± 0.01

1.83**

1.25**

Glucose−6 − phosphate

0.03 ± 0.00

0.14 ± 0.04

0.95 ± 0.15

1.55 ± 0.22

2.49*

0.70

Organic acid

Lactic acid

2.17 ± 0.21

4.79 ± 0.91

0.69 ± 0.09

0.93 ± 0.17

1.15*

0.44

Galactonic acid

0.63 ± 0.08

2.08 ± 0.25

1.05 ± 0.35

1.42 ± 0.14

1.72**

0.44

Glyceric acid

8.33 ± 0.94

8.12 ± 2.57

9.87 ± 0.91

15.71 ± 2.56

−0.04

0.67*

Pyruvic acid

7.98 ± 0.17

2.84 ± 0.71

0.34 ± 0.08

0.85 ± 0.11

−1.49**

1.31*

Fumaric acid

44.89 ± 4.92

7.64 ± 1.23

0.06 ± 0.02

0.07 ± 0.01

−2.55**

0.11

Malic acid

0.16 ± 0.01

0.11 ± 0.01

3.39 ± 0.57

5.29 ± 1.30

−0.50*

0.64

Citramalic acid

0.35 ± 0.06

0.23 ± 0.02

0.16 ± 0.00

0.26 ± 0.04

−0.62

0.71*

Succinic acid

7.73 ± 0.72

7.15 ± 1.28

6.33 ± 1.30

10.06 ± 0.74

−0.11

0.67*

Ketoisocaproic acid

0.02 ± 0.00

0.03 ± 0.00

11.15 ± 0.46

22.50 ± 0.38

0.55**

1.01**

2 − Deoxytetronic acid

18.32 ± 3.67

30.44 ± 2.30

3.47 ± 0.61

9.71 ± 1.54

0.73

1.49

Oxalic acid

0.19 ± 0.03

0.24 ± 0.04

0.12 ± 0.01

0.26 ± 0.04

0.71

2.26*

Propionic acid

0.14 ± 0.03

0.17 ± 0.01

0.83 ± 0.20

3.03 ± 0.34

0.34

1.13**

Maleic acid

14.74 ± 1.66

7.75 ± 1.14

1.73 ± 0.20

9.20 ± 3.54

0.28*

1.86

Gluconic acid

0.11 ± 0.01

0.12 ± 0.02

0.10 ± 1.03

0.34 ± 0.09

−1.10

0.58*

Glucoheptonic acid

1.98 ± 0.13

2.64 ± 0.20

1.71 ± 0.49

3.57 ± 0.71

−0.93

2.41

Fatty acids

Butyrate

0.31 ± 0.01

0.15 ± 0.07

25.66 ± 1.03

38.35 ± 2.81

0.13

1.83*

Isobutyric acid

0.26 ± 0.05

0.43 ± 0.04

2.53 ± 0.38

12.14 ± 3.61

0.41

1.06

Stearic acid

0.16 ± 0.01

0.42 ± 0.07

1.92 ± 0.10

3.50 ± 0.53

1.43*

0.87*

Linoleic acid

0.01 ± 0.00

0.02 ± 0.00

8.30 ± 2.49

22.03 ± 0.87

0.83*

1.41**

Linolenic acid

0.34 ± 0.03

1.14 ± 0.16

3.19 ± 0.40

2.49 ± 0.33

1.75**

−0.36

Palmitic acid

0.55 ± 0.04

1.64 ± 0.22

0.27 ± 0.02

0.82 ± 0.26

1.56**

1.59

Butyric acid

0.31 ± 0.22

0.14 ± 0.01

0.95 ± 0.10

0.67 ± 0.04

−1.09

−0.52*

Phenylpropanoids

Neohesperidin

0.81 ± 0.11

2.14 ± 0.45

0.17 ± 0.03

1.27 ± 0.24

1.40*

2.90**

Salicylic acid

1.23 ± 0.00

1.60 ± 0.17

2.28 ± 0.24

7.70 ± 1.42

0.38

1.76**

Trans − cinnamate

0.36 ± 0.03

1.08 ± 0.20

1.43 ± 0.11

4.59 ± 0.74

1.59*

1.68**

Naringin

0.01 ± 0.00

0.06 ± 0.02

0.78 ± 0.24

1.81 ± 0.59

3.04*

1.21

Ferulic acid

0.81 ± 0.08

1.16 ± 0.05

0.47 ± 0.04

0.31 ± 0.03

0.52*

−0.61**

Caffeic acid

0.01 ± 0.00

0.02 ± 0.00

6.48 ± 0.68

5.12 ± 0.54

1.16

−0.34

Prunin

0.29 ± 0.02

0.19 ± 0.07

2.41 ± 0.51

5.61 ± 0.19

−0.61*

1.22*

  1. Relative concentrations and standard deviation were increased by a factor of 100 times in each treatment. Values were presented as the mean ± standard error of four biological replicates. C Cultivar soybean, W Wild soybean, CK Control treatment, LN Low−N stress; * and ** indicate significant (P < 0.05) and highly significant (P < 0.01) differences, respectively