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Fig. 4 | BMC Plant Biology

Fig. 4

From: NAL8 encodes a prohibitin that contributes to leaf and spikelet development by regulating mitochondria and chloroplasts stability in rice

Fig. 4

NAL8 contributes to localized cell proliferation and cell size. a The visual splices of leave cross-section surface of TQ. Scale bar = 200 μm. b Enlargement of the region outlined in blue in a showing the TQ vascular bundles. Scale bar = 100 μm. c The visual splices of leave cross-section surface of nal8 mutants. Scale bar = 200 μm. d Enlargement of the region outlined in red in b showing the vascular bundles in the nal8 mutant. Scale bar = 100 μm. The arrows indicate the cell division aberration in the nal8 mutant. The visual splices were performed by X-Ray microscope from Zeiss company. e-h Statistical analyses of vascular bundle number (e), leaf thickness (f), xylem cell number (g), and xylem cell length (h) in TQ and the nal8 mutant based on X-ray microscope observation (n = 3 leaves). Values are given as the mean ± SD. **P < 0.01 compared with the TQ control using Student’s t-test. i Flow cytometry analysis of root tip (1–3 cm) cells from TQ and the nal8 mutant. The two peaks represent 2C and 4C nuclei, respectively. j Percentage of cells at different phases of the cell cycle in young root tip cells (n = 10 pooled tissues, and three young root tips from different plants per pool). The G1, S, and G2/M phases are shown by colored boxes. k Relative expression levels of the genes involved in the cell cycle in TQ and the nal8 mutant. The UBQ5 gene was used as an internal reference to normalize the gene expression data. Values in e-h, j, k are given as the mean ± SD. *P < 0.05; **P < 0.01 compared with the TQ control using Student’s t-test

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