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Fig. 6 | BMC Plant Biology

Fig. 6

From: Identification of heat-tolerance QTLs and high-temperature stress-responsive genes through conventional QTL mapping, QTL-seq and RNA-seq in tomato

Fig. 6

The heat map analysis of the expression of 25 DEGs underlying the major QTLs. The average log signal expression values of genes in various times and parents were denoted at the top with a color scale, in which green, black and red color indicated the low, medium and high level of expression, respectively. The samples and genes used for expression profiling were indicated on the top and right side of the heat map. The expression level of 0 h of parents was used as control and actin was used as the endogenous control in qRT-PCR analysis. The expression level of genes calculated by three independent biological replicates with three technical replicates in qRT-PCR assay. Twelve candidate genes showing pronounced differential expression in parents during high-temperature stress were detected. Four genes (Gene ID numbers were: 101252525, 101,265,863, 101,251,744, and 543,944) were further screened from the 12 candidate genes by combining GO and KEGG function analysis: SlGST, SlUBC5, SlCathB2, and SlARG1

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