Identification of heat-tolerance QTLs and high-temperature stress-responsive genes through conventional QTL mapping, QTL-seq and RNA-seq in tomato

Table 2 Conventional QTL analysis for heat tolerance-related physiological indexes in the F₂ population

Trait	CH	QTL^a	Peak Position (cM)^b	Flanking markers^c	Location (Mb)^d	LOD^e	PVE (%)^f	Add^g
REC	1	qREC-1-1	52.0	W299-SL20134_408i	24.10–80.54	11.59	6.70	−0.13
	1	qREC-1-2	71.0	SL20134_408i-SSR270	80.54–80.70	10.62	5.81	−0.17
	1	*qREC-1-3*	156.0	*SSR134 -C01M86371*	81.64–86.37	3.78	5.75	−0.15
	2	qREC-2-1	47.0	SSRD69-C02M48329	43.48–48.33	5.41	5.66	−0.19
	3	qREC-3-1	119.0	C03M68619-C03M70037	68.62–70.04	3.24	1.04	−0.05
	9	qREC-9-1	18.0	SSRD133-TGS1354	1.11–4.01	2.60	2.14	0.07
	12	qREC-12–1	13.0	SL10953_259i-SSRD80	3.79–36.67	9.13	5.70	0.18
CC	1	qCC-1-4	118.38	C01M75064-SSR134	75.06–81.64	3.70	1.71	−0.06
	1	*qCC-1-5*	160.66	*SSR134 -C01M86371*	81.64–86.37	3.72	16.48	0.22
	2	qCC-2-2	0.01	SSR96-C02M4005	39.71–40.00	3.52	4.86	0.05
F_v/F_m	5	q F_v/F_m − 5-1	1.51	SSR13-SSR115	2.53–2.73	2.50	6.94	0.18
F_v/F_m	12	qF_v/F_m − 12–2	80.29	SSRD74- LeOH301	54.37–63.56	3.00	8.80	0.03

REC indicates relative electrical conductivity, CC indicates chlorophyll content, F_v/F_m indicates maximum photochemical quantum efficiency
The consistent QTLs qCC-1-5 and qREC-1-3 on chromosome 1 are shown in bold
^athe QTLs for REC, CC and F_v/F_m are temporarily named ‘qREC, qCC and qF_v/F_m + number of chromosome + number of QTL’
^bThe position of heat tolerance-related QTLs
^cFlanking markers of heat tolerance-related QTLs
^dThe physical position of flanking markers linked with heat tolerance QTLs
^ePeak LOD value of the QTL
^fThe phenotypic variation explained by the putative QTL
^gAdditive effect. A positive value indicates that the genes derived from heat tolerance parent LA2093 contributed to increased heat tolerance, and the negative value indicates that genes originated from LA1698 reduce the effect on tomato heat tolerance

ISSN: 1471-2229