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Fig. 1 | BMC Plant Biology

Fig. 1

From: GhFAD2–3 is required for anther development in Gossypium hirsutum

Fig. 1

The expression profiles of GhFAD2 genes in different tissues and at different developmental stages of cotton seed and fiber. Total RNA was isolated from root, leaves, stem, anther, stigma, ovary, seed and fiber. Each sample had three biological replicates. The seeds at 40 DPA and fiber at 24 DPA were used: In seeds, the expression level of GhFAD2 reached its peak at 40 DPA [12]. It has been difficult to identify changes in gene expression for fiber after 30 DPA due to hard to extract mRNA. For each sample, a total of 3 μg RNA was used in preparing the RNA-seq library. Barcoded multiplexed RNA-seq libraries were created using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) according to the manufacturer’s protocol. Clean paired-end reads were aligned to the TM-1 reference genome, and the number of reads aligned to each gene was measured using HTSeq v0.6.1.The expression levels of individual genes were quantified using FPKM. Error bars are standard errors. Genes with an adjusted P-value < 0.05 found by DESeq were assigned as differentially expressed. * indicates a differentially expression of GhFAD2–3D between anther and other tissues (Padj < 0.05)

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