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Fig. 7 | BMC Plant Biology

Fig. 7

From: GhFAD2–3 is required for anther development in Gossypium hirsutum

Fig. 7

qRT-PCR analysis of some candidate genes involved in the biosynthetic pathways for cutin monomers in in wild-type and fad2–3. St1-St5: Anther at different developmental stages; St1, Sporogenous cell stage; St2, Microsporocyte stage; St3, Meiosis stage; St4, Tetrad stage; St5, Pollen maturation stage. The GhFAD2–1, GhFAD3, GhSAD, GhCYP704B1, GhCYP86B1, GhHTH, GhALDH, GhPXG, and GhCYP94C1 mRNA abundance was determined by qRT-PCR, respectively. These genes encodes enzymes indicated as follows: Fatty acid desaturase 2, Fatty acid desaturase 3, Δ9-stearyl-ACP desaturase, Cytochrome P450 704B1, Cytochrome P450 86B1, ω-hydroxyacid dehydrogenase, aldehyde dehydrogenase, peroxygenase and Cytochrome P450 94C1. GhUBQ14 was used as a reference gene. All qRT–PCR reactions were performed in triplicate. Relative gene expression levels of target genes were normalized against Ct values for GhUBQ14, and the fold change (2–ΔΔCt) was determined by comparison to average expression levels. Significant differences from control were marked with * (P < 0.05), ** (P < 0.01) and *** (P < 0.001)

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