Fig. 8
Complementation of the 637ys mutant with OsLIL3 gene. a Identification of transgenic plants by PCR. M, DL 2000 marker; 1, pC2300-OsLIL3 plasmid (positive control); 2, 637ys (negative control); 3–5, positive transgenic plants. b and c Phenotypes of wild-type (WT), 637ys and PCR-positive transgenic plants (TP) at the seedling stage and at the grain-filling stage, respectively. d, e and f The elution profiles of Chl in leaves of WT, 637y, and TP at the seedling stage were detected at 660 nm, respectively. Peaks 2, 3, 4, and 5 represent Chlphy a, ChlTHGG a, ChlDHGG a, and ChlGG a, respectively. Peaks 1, 6 and 7 represent Chlphy b, ChlDHGG b, and ChlGG b, respectively. The absorption spectra of elution profiles in acetone are the same as those in Additional file 1: Figure S1c and d. g, h and i The elution profiles of the tocopherols in leaves of WT, 637ys, and TP at the seedling stage were detected, respectively, by fluorescence with excitation at 290 nm and emission at 330 nm. Peaks 1 and 2 represent α-tocopherol and γ-tocopherol, respectively, and Peak 7 might be the isomer of γ-tocopherol