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Fig. 1 | BMC Plant Biology

Fig. 1

From: A modular steroid-inducible gene expression system for use in rice

Fig. 1

Schematic illustration of the constructs generated in this study. Plasmids 17203, 17610 and 17613 were created using the Golden Gate modular cloning system (see Additional files 234 for complete sequence of each). HYG: hygromycin phosphotransferase; p35S: CaMV 35S promoter; pOsAct: rice actin promoter; pZmUbi: maize ubiquitin promoter; GUS: uidA gene encoding β-glucuronidase. The inducible system consists of the activator, i.e. a rice codon optimized (rco) version of LhGR plus its cognate pOp6 promoter. In each construct, all four promoter:coding sequence modules contain the nos terminator (not represented)

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