Fig. 6

Addition of IPTG to the growth medium does not inhibit activation of pOp6-driven reporter gene expression. Seedlings shown in Fig. 4 stained for GUS activity. a-c) pZmUbi:GUS and wild-type controls. d-f) T1 seedlings from independent inducible lines. All seeds were germinated on ½ MS plates for 3 days and then transferred to plates containing either: 10 mM IPTG (a, d): 10 μM DEX (b, e) or 10 μM DEX plus 10 mM IPTG (c, f) and grown for 5 days. Some lines segregated non-transgenic siblings which were not affected by DEX-mediated LhGR activation (marked by red arrows). Scale bar = 1 cm