Fig. 8
From: A modular steroid-inducible gene expression system for use in rice
Reporter gene expression can be induced by seedling submergence. a-c) GUS activity in representative 7 day old seedlings that were germinated and grown on ½ MS plates before submergence in non-inductive (0.1% DMSO and 0.1% Tween-20) (a, b) or inductive (10 μM DEX, 0.1% Tween-20) (c) solutions for 19 h. D-F) Phenotype of plants transferred to hydroponic growth conditions for 5 days after submergence treatment. Prior to submergence in non-inductive (d) or inductive (e, f) conditions, plants had been grown in either ½ MS (d, e) or in ½ MS media containing 10 mM IPTG. Red arrows indicate segregating wild type plants. Scale bar = 1 cm