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Table 1 Occurrence of lacO-related sequences in the rice genome

From: A modular steroid-inducible gene expression system for use in rice

lacI wild type binding sites

number of hits in the rice genome (putative promoters)

exact matches

3 mismatches

4 mismatches

5 mismatches

lacO1 AATTGTGAGCGGATAACAATT

1

4

111 (4)

1144 (43)

lacO2 AAATGTGAGCGAGTAACAACC

0

6 (1)

88 (3)

709 (35)

lacO3 GGCAGTGAGCGCAACGCAATT

1

3

53 (3)

436 (38)

  1. The three E. coli lacO sequences were used to query the rice genome, with criteria set to return all hits with a maximum five mismatches (all of which are predicted to bind lacI – based on binding studies in tobacco ([22])