Fig. 1From: Silencing of one copy of the translation initiation factor eIFiso4G in Japanese plum (Prunus salicina) impacts susceptibility to Plum pox virus (PPV) and small RNA productionProduction and sharka resistance of transgenic Japanese plum lines. a Schematic representation of the RNAi constructs used for Japanese plum transformation. PCR fragments of PpeIF4E, PpeIFiso4E, PpeIF4G, PpeIFiso4G10, and PpeIFiso4G11 were cloned in opposite orientations as indicated by hatched arrows. b Assessment of resistance to PPV infection. Values represent the mean optical density values of three to four replicates per transgenic line tested for PPV infection over 3 to 5 vegetative cycles. Dotted line shows the basal OD value of the healthy negative control (‘Angeleno’). AG: ‘Angeleno’ transgenic lines; LA: ‘Larry Ann’ transgenic lines. Numbers starting with 73 were transformed with pH 12-PpeIFiso4G11; 74 with pH 12-PpeIFiso4G10; 72 with pH 12-PpeIF4G and 53 with pBINPLUS/ARS-PpeIF4E. All plants were grafted on rootstocks infected with PPV-M except NI (non-infected) and the plants noted PPV-D (PPV-D8 and PPV-D RdF Rouge de Fournés isolates). c Absence of symptoms on PpeIFiso4G11-silenced (left) and PPV symptoms on infected wild-type ‘Angeleno’ (right) Japanese plums. The susceptible plant on the right shows wilting of the bud leaves. d Close-up of PPV-infected ‘Angeleno’ leaf displaying chlorotic symptoms and vein clearingBack to article page