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Fig. 2 | BMC Plant Biology

Fig. 2

From: Silencing of one copy of the translation initiation factor eIFiso4G in Japanese plum (Prunus salicina) impacts susceptibility to Plum pox virus (PPV) and small RNA production

Fig. 2

Relative expression analysis of PpeIFiso4G11 and PDK intron in leaves of transgenic Japanese plum trees. Transcript levels were analyzed by qRT-PCR. All values were normalized to the TEFII reference gene and then compared to the wild type ‘Angeleno’ gene expression level. Error bars represent the standard deviation of two biological replicates each analyzed in triplicate. AG: transgenic ‘Angeleno’ plum lines; LA: transgenic ‘Larry Ann’ plum lines. All plants were grafted on rootstocks infected with PPV-M except NI (non-infected). The transgenic line numbers are corresponding to the ones depicted in Fig. 1b. The standard deviation between replicates is indicated by vertical lines. Statistical analysis was performed using the Kruskal–Wallis rank sum test in R software v. 3.2.5. Significantly different values are noted with lowercase letters (P value ≤0.05) when comparing the expression level between transgenic and wild-type lines. a qRT-PCR estimation of the PpeIFiso4G11 transcript levels in transgenic Japanese plum lines. b Transcriptional expression of the PDK intron in transgenic Japanese plum lines. Note that only the pH 12-PpeIF4G, pH 12-PpeIFiso4G10 and pH 12-PpeIFiso4G11 transformed lines were tested here

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