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Fig. 4 | BMC Plant Biology

Fig. 4

From: Silencing of one copy of the translation initiation factor eIFiso4G in Japanese plum (Prunus salicina) impacts susceptibility to Plum pox virus (PPV) and small RNA production

Fig. 4

Model of eIFiso4F-mediated susceptibility to PPV in diploid plum. a In diploid Prunus species, the eIF4F translation initiation complex is composed of eIF4E which interacts with the mRNA (m7Gppp) cap, eIF4G which interacts with both eIF4E and the polyadenosine-bound PolyA binding proteins (PABP), and eIF4A. On the other hand, two copies of eIFiso4G (PpeIFiso4G10 and PpeIFiso4G11) are transcribed and the corresponding proteins are both able to form the eIFiso4F complex, in interaction with a single PpeIFiso4E isoform, PABP and eIF4A (A, left panel). In this model, the only eIF4G and isoform used by PPV is PpeIFiso4G11. In non-transgenic, wild type plum infected with PPV, interaction of the viral genome-linked protein, VPg, with the eIFiso4F complex that involves PpeIFiso4G11 is leading to plant susceptibility (A, right panel, in red). The viral RNA genome is represented in red and the host mRNA, in blue. b In PpeIFiso4G11-silenced plum, host cell messenger RNAs are still recruiting the eIF4F or/and eIFiso4F complex that involves PpeIF4G and PpeIFiso4G10, respectively (B, left panel), thus preventing abnormal growth of the plant. However, the virus is no longer able to hijack the eIFiso4F complex because of the absence of PpeIFiso4G11 factor (B, right panel). It results in a failing viral cycle, the virus being impaired either in its translation, replication and/or cell-to-cell movement

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