Fig. 1

2DE analysis of MOAC-enriched putative phosphoproteins from rice leaves. Original-color image of the same 2DE gel was stained by a Pro-Q diamond and b silver nitrate. c Venn diagram analysis of MOAC-enriched putative phosphoproteins in 2DE gels that overlapped between Pro-Q Diamond staining (green) and silver staining (red). False-color images of 2DE gels were visualized with different colors using PDQuest software; d Pro-Q diamond-stained protein spots were colored green and e silver-stained protein spots were colored red. f An overlay of the two images (d and e). The phosphoprotein spots were appeared yellow, and the non-phosphoprotein spots were appeared red (as shown by arrows). Asterisks indicate spots that were very abundant in the silver image but lightly stained in the Pro-Q Diamond image