Fig. 6

Silencing GmWRKY40 decreased the accumulation of ROS following P. sojae infection. a Accumulation of H₂O₂ was detected by DAB staining at 6, 12 and 24 h after inoculated with P. sojae. This experiment was repeated three times with similar results. b H₂O₂ content was measured at 6, 12 and 24 h after inoculated with P. sojae. Values represent the means of 3 replicates ± SD. Asterisks indicate statistically significant differences (*P < 0.05 or **P < 0.01). c-e Transgenic hairy roots were inoculated with zoospore suspension of P. sojae and samples were collected at 0 or 24 h after inoculation. Gene expression of GmNADPHox, GmAPX1 and GmCAT1 were analyzed by qRT-PCR. Values represent the means of 3 replicates ± SD. ^** Student’s t-test: p < 0.01